Preparation of substituted perhydroindenes and perhydronapthalenes

ABSTRACT

3-SUBSTITUTED-6AB-ALKYL-4A-HYDROXY, ALKOXY, AND ACLOXY PERHYDROCYCLOPENTA(F)(1)BENZOPYRANS OR PERHYDRONAPH THO(2,1-B)PYRANS ARE CONVERTED INTO 4-(3-OXO-ALKYL)PERHYDROINDENE 5-ONES OR THE CORRESPONDING PERHYDRONAPHALENE-6-ONES. THESE LATTER COMPOUNDS CAN BE CONVERTED TO KNOWN STERODIAL MATERIALS WHICH ARE PHARMACOLOGICALLY ACTIVE AS PROGESTATIONAL AGENTS.

United States Patent Oflice 3,576,006 Patented Apr. 20, 1971 US. Cl. 260340.9 9 Claims ABSTRACT OF THE DISCLOSURE 3-substituted-6ocB-alkyl-4a-hydroxy, alkoxy, and acyloxy perhydrocyclopenta[f] [1]benzopyrans or perhydronaphtho[2,l-b]pyrans are converted into 4-(3-oXo-alkyl)perhydroindene-S-ones or the corresponding perhydronaphalene-6-ones. These latter compounds can be converted to known steroidal materials which are pharmacologically active as progestational agents.

RELATED APPLICATIONS This application is a division of applicants copending application Ser. No. 679,989, filed Nov. 2, 1967, which is a continuation-in-part of applicants copending application Ser. No. 633,730, filed Apr. 26, 1967, which was filed as a continuation-in-part of applicants copending application Ser. No. 604,124, filed Dec. 23, 1966, now abandoned, which was filed as a continuation-in-part of applicants copending application Ser. No. 549,816 filed May 13, 1966, now abandoned.

BACKGROUND OF THE INVENTION The reactants and products produced in accordance with l the teachings of this invention are valuable as intermediates in the total synthesis of steroids. Total syntheses utilizing these compounds as intermediates are described in US. patent applications of Gabriel Saucy, Ser. No. 549,816, filed Dec. 23, 1966, now abandoned; Ser. No. 604,124, filed May 13, 1966, now abandoned; Ser. No. 633,730, filed Apr. 26, 1967 and Ser. No. 679,989, filed Nov. 2, 1967. Other related applications include Ser. No. 633,693, filed Apr. 26, 1967, now abandoned.

DETAILED DESCRIPTION OF THE INVENTION This invention is concerned with certain polycyclic compounds and with processes for their synthesis. More particularly, this invention relates to novel cyclopenta- [f] [1]-benzopyrans and 7H-naphtho[2,1-b]pyrans, and to methods for their production. These compounds are useful as intermediates in syntheses of steroids and iD-homosteroids, respectively. In syntheses of steroidal materials steric considerations are of great significance. The most used steroidal compounds are those having a C/D-trans ring junction with the substituent in the l3-position being in the fi-stereoconfiguration. The present invention provides a facile total synthesis of 13B-C/D-trans-steroidal materials. This desirable result is obtained via a unique asymmetric induction with optical specificity preserved in subsequent reaction steps.

In a major aspect, this invention is concerned with novel cyclopenta[f] [l]benzopyrans having the tricyclic nucleus and novel naphtho[2,1-b]pyrans having the tricyclic nucleus These novel compounds are generally defined by the formula:

gat

wherein Y is a 3-oxobutyl R14) CH(R15 or R; R is hydrogen, an alkyl group of from 1 to 6 carbons, or a 3-substituted butyl radical of the formula R5CH2C(R3,R4)3CH(R14)CH(R15) R1 IS a primary alkyl group of from 1 to 5 carbon aotms; R is hydrogen, lower primary alkyl, or lower acyl; R when taken alone, is -OR R when taken alone, is hydrogen; R and R when taken together, are lower alkylenedioxy, the monothia, monoaza or dithia chalcogen thereof; R R R R and R are each independently hydrogen or lower alkyl; R is hydrogen, lower alkyl, lower acyl or lower oxyhydrocarbyl; Z is carbonyl or a group of the formula R is hydrogen or lower acyl; R is hydrogen or lower aliphatic hydrocarbyl; T represents either a single or a double bond; U represents a single or a double bond and is a single bond when T is a single bond; m is an integer having a value of from 1 to 2; n is an integer having a value of from 0 to 1 and is 0 when T represents a double bond and is 1 when T represents a single bond; r is an integer having a value of from 0 to 1 and is 0 when T is a double bond and 1 when T is a single bond; and s is an integer having a value of from 0 to l and is 0 when U is a double bond and 1 when U is a single bond.

As used throughout the specification and appended claims, the term hydrocarbyl group denotes a monovalent substituent consisting solely of carbon and hydrogen; the term hydrocarbylene denotes a divalent substituent consisting solely of carbon and hydrogen and having its valence bonds from different carbons; the term aliphatic, with reference to hydrocarbyl or hydrocarbylene groups, denotes groups containing no aromatic unsaturation, but which can be otherwise saturated or unsaturated, i.e., an alkyl or alkylene, or an aliphatic group containing olefinic or acetylenic unsaturation; the term alkyl group denotes a saturated hydrocarbyl group, whether straight or branched chain; the term primary alkyl group denotes an alkyl group having its valence bond from a carbon bonded to at least two hydrogens; the term acyl group denotes a group consisting of the residue of a hydrocarbyl monocarboxylic acid formed by removal of the hydroxyl portion of the carboxyl group; the term oxyhydrocarby denotes a monovalent saturated cyclic or acyclic group consisting of carbon, hydrogen, and oxygen containing only one oxygen in the form of an ether linkage; and the term lower, as applied to any of the foregoing groups, denotes a group having a carbon skeleton containing up to and including eight carbons, such as methyl, ethyl, butyl, tert.-butyl, hexyl, Z-ethylhexyl, vinyl, butenyl, hexenyl, ethinyl, ethylene, methylene, formyl, acetyl, 2-phenylethyl, benzoyl, methoxymethyl, l-methoxyethyl, tetrahydropyran-Z-yl, and the like. The phraseology lower alkylenedioxy, the monothia, monoaza or dithia chalcogen thereof is used to indicate a ketalized x0 or thio moiety and comprehends moieties of the formula -OR O, OR S, -OR N or AR S wherein R is lower alkylene. Exemplary moieties are 1,2-ethylenedioxy, 2,2-dimethyl 1,3 propylenedioxy, 1,2-ethyl'enedimercapto, 2,3- butylenedioxy and the like.

In the formulas presented herein, the various substituents on cyclic compounds are joined to the cyclic nucleus by one of three notations, a solid line indicating a substituent which is in the IS-orientation (i.e., above the plane of the paper), a dotted line indicating a substituent which is in the a-orientation (below the plane of the paper), or a Wavy line indicating a substituent which may be in either the aor ,B-orientation. The position of R has been arbitrarily indicated as the fi-orientation, although the products obtained in the examples are all racemic compounds unless otherwise specified.

Preferred compounds are those wherein Y is R; R is n-alkyl, especially methyl, 3,3-(alkylenedi0xy)butyl wherein the alkylenedioxy group, when taken with the 3-carbon of the butyl radical, forms a dioxolane ring system, especially 3,3-(ethylenedioxy)butyl and 3,3-(2,3-butylenedioxy)butyl; 3-hydroxybutyl, 3-tert.-alkoxybutyl, especially -tert.-butoxybutyl, or 3- (tetrahydropyran-2-yloxy) butyl; R is n-alkyl, especially methyl and ethyl; and, when s has a value of 1, the 9a-(When m is 1) or 100t-(Wh6n m is 2) hydrogen is trans-oriented with respect to R Subgeneric to the tricyclic compounds of Formula I are the 3-substituted-6aB-alkyl-l,2,3,5 ,6,6a,7, 8-octahydrocyclopenta[f] [llbenzopyrans (by alternate nomenclature 3- substituted-6a 9-alkyl 2,3,5,6,6a,8 hexahydro-lH-cyclopenta[f] [11-benzopyrans) and the 3-substituted-6afl-alkyll,2,5,6,6a,7,8,9-octahydro 3H-naphtho[2,l-b]pyrans (by alternate nomenclature 3-substituted-6afl-alkyl-1,2,3,5,6, 6a,8,9-0ctahydro 7H-naphtho [2,l-b] pyrans), hereinafter referred to as dienes, having the formula:

R12 Ia wherein R R 2, Z, Y, and m are as defined above;

the 3-substituted 6215 alkyl-l,2,3,5,6,6a,7,8,9,9a-decahydrocyclopentaff] [l]benzopyrans (by alternate nomenclature 3-substituted 6afi1 alkyl-2,3,5,6,6a,8,9,9a-octahydro- 1H-cyclopenta[f][l]benzopyrans) and the 3-substituted- 6a,B-alkyl-l,2,5,6,6a,7,8,9,l0,10a decahydro-BH-naphtho- [2,1-b1pyrans (by alternate nomenclature 3-substituted- 6a,!3-alkyl-l,2,3,5,6,6a,8,9,10,10a decahydro-7H-naphtho- [2,1-b1pyrans), hereinafter referred to as monoenes, represented by the formula:

YCHM U u 3 R12 Ie wherein R R R R Z, Y, and m are as defined above.

Alternatively, the tricyclic compounds of Formula I can be classified according to the nature of Y, which determines the utility of the compounds of this invention. The first of these classes are the 3-alkyl compounds of the formula:

wherein R R R R Z, m, n, r, s, T, and U are as defined above and x is an integer having a value of from 0 to 6, inclusive.

These products are useful as intermediates for the synthesis of members of a recently discovered class of 9p, lOocor retrosteroids, and also are useful as intermediates for the synthesis of wot-steroids, and other steroidal materials.

The second class of intermediates classified according to utility are the 3-(4-substituted pen'tyl) compounds of the formula:

RaCHgACHCHCHg M M3,,

wherein R1, R2, R5, R11, R12, R14, R15, Z, m, n, r, S, T, and U are as defined above; A is carbonyl or CR R and R and R are as defined above.

These compounds, which are useful as intermediates f r the synthesis of 19-nor-steroids f the normal series, and other steroidal materials, can be further classified as:

1) The 3-(4-oxopentyl)-substituted cyclopentabenzopyrans and naphthopyrans of the formula:

O (R O) m T R CH ECHCHCH i i 1;

| I R14 R15 R12 Ie-l wherein R1 2 5 11 12 14 15, T, and

U are as defined above.

(2) The 3-[4,4-(alkylenedioxy)pentyl]-substituted cyclopentabenzopyrans and naphthopyrans and side chain chalcogens of the formula:

R14 Ru: 3 Rn wherein 1 2 5 9 11, 12 14 15, S, T, and U are as defined above; and both hs are either sulfur or oxygen, or one h is oxygen and the other is sulfur or nitrogen. and

(3) The 3-(4-hydroxypentyl)-substituted cyclopentabenzopyrans and naphthopyrans and ethers thereof of the formula:

wherein R R R R R R R R Z, m, n, r, s, T,

and U are as defined above.

In compounds of formula Ie-4, R as t-butyl is especially preferred.

In a second aspect, this invention is concerned with a method for producing the compounds of Formula I via the following general reaction scheme:

II YCHgCHCHCHCH2CCH=CH2 H2)m II 0 III wherein Y, R R R R Z, and m are as defined above; and B is hydrogen, lower alkyl or lower acyl.

Thus, the process of this invention comprises the general steps of (l) condensation of a 7-hydroxy-1-alken-3- one or a variant thereof (II), as defined below, with a 2-alkylcycloalkane-1,3-dione (III), as defined below, to produce diene (Ia); (2) saturation of the 9,9aor 10,10adouble bond of diene (Ia) to produce monoene (Ib); and (3) introduction of a hydroxy, alkoxy, or acyloxy group at the 4a-position and a hydrogen atom at the 9bor l0b position of monoene (Ib) to produce perhydro compound (Ic). It is to be understood that the foregoing reaction sequence is merely schematic in nature, and that each depicted step can represent only one or more than one reaction, as will be more fully described herein.

It will be noted that the diene, monoene and perhydro compounds of this invention can bear a.3-(4-oxopentyl)- substituent. When such a side chain is desired, it is however preferable to perform the reaction sequence with compounds having the 0x0 moiety of the 4-oxopentyl side chain in protected form. Protection can be effected by ketalization (to form lower alkylenedioxy, the monothia, monoaza or dithia chalcogen thereof), or by reduction to a hydroxy moiety optionally followed by etherification or esterification. The oxo moiety can be regenerated at any intermediate stage as desired.

1-alken-3-one compounds of Formula II are employed as one of the starting materials for the foregoing reaction sequence. Illustrative examples of these 1-alken-3- ones include 7-hydroxy-1-octen-3-one, 7-hydroxy-1-nonen- 3-one, 7-hydroxy-1-dodecen-3-one, 7-acet0xy-1-nonen-3- one, 7-benzoyloxy-l-nonen-3-one, 7-methoxy-1-nonen-3- one, 7-benzyloxy-1-nonen-3-one, 11,1l-ethylenedioxy-7- hydroxy-1-dodecen-3-one, 7,ll-dihydroxy 1 dodecen-3- one, 1l-tert.-butoxy-7-hydroxy-1-dodecen-3-one, ll-(tetrahydropyran-Z-yloxy)-7-hydroxy-1-dodecen-3-one, and the like.

7 The 7-hydroxyalken-3-ones of Formula II above are readily synthesized from (A) a glutaric acid anhydride, (B) a 2-alkylcyclohexane-1,3-dione, (C) a glutaraldehyde or (D) a butyrolactone, as is illustrated by the following sequences leading to 7-hydroxynonen-3-ones:

O O i] ll 0: E 0: MAR 0:

0 O g l HO (EHGHCHz O-alkyl z alky CHE (IJHCHCH o 1 0 II n I RCHzC CHCHCHZAJ 0-alky1 non R YCHz The preparation of starting materials useful in the practice of the present invention is described in greater detail in United States patent application of David A. Andrews and Gabriel Saucy, Ser. No. 633,693, filed Apr. 26, 1967, entitled, 6-Substituted Tetrahydropyran-Z-Ols and Process for Their Preparation; and in US. patent application 'Ser. No. 604,124, filed Dec. 23, 1966 entitled a-Olefins.

The dienes of Formula Ia in the presence of Water and acid, e.g., sulfuric acid in acetone, aqueous acetic acid or aqueous hydrochloric acid in dioxane, undergo acid hydrolysis to form indenones of the formula Rn HO g R12 Ia wherein R R11 R Y and m have the same meaning as above.

The indenones of Formula Ia are themselves convertible to compounds of Formula Ia via dehydration, for example, via acid catalyzed azeotropic distillation in benzene. Suitable acid catalysts are p-toluenesulfonic acid, potassium bisulfate, boron trifluoride etherate and the like. This reversible hydrolysis of compounds of Formula la is useful in their preparation and purification. Thus, in instances where the direct purificaton of compounds of Formula Ia is difiicult it is often more facile to hydrolyze the compound of Formula Ia to a compound of Formula Ia, which can then be purified, for

O u U M I? ClCCHCHGHzCl IV 12 R11 OH l I ll YCHzCHCHCHCHzC OH=CH2 R 2 Ru MAR 11 example, by chromatography, and subsequently be reconverted to the desired compound of Formula Ia via dehydration.

The ketodienes of Formula Ia-l are readily converted to the corresponding 7,8-alcohols and their esters as represented by the formula:

R12 Ia-2 wherein Y, R R R11, R and m are as previously defined,

clusive, i.e., boron and aluminum. Illustrative examples of these reducing agents include an alkali metal, preferably lithium or sodium, in liquid ammonia or a liquid aliphatic amine; tri(lower alkoxy)-aluminum compounds such as trisopropoxyaluminum; di(lower alkyl)-aluminum hydrides such as diethylaluminum hydride and diisobutyl-aluminum hydride; alkali metal-Group III-metal complex hydrides such as lithium aluminum hydride, sodium aluminum hydride, and sodium borohydride; tri- (lower alkoxy)alkali metal-Group III-metal complex hydrides such as trimethoxy lithium aluminum hydride and tributoxy lithium aluminum hydride; diisobutyl aluminum hydride and the like. The alkali metal-Group III- metal complex hydrides are preferred as reducting agents, with the nonalkaline reagents, such as lithium aluminum hydride, being especially preferred.

This reaction is effected in any suitable inert reaction medium, such as hydrocarbons, e.g., cyclohexane, benzene, toluene, and xylene; ethers, e.g., diethyl ether, diisopropyl ether, and tetrahydrofuran. Protic solvents, such as water or alcohols, should not be employed when lithium aluminum hydride is the reducing agent, but can be employed with sodium borohydride.

The remaining reaction conditions are not narrowly critical, although it is generally preferred to effect the reduction at reduced temperatures, i.e., below about room temperature (about 20-25 C.). Temperatures in the range of from about C. to about room temperature are normally employed.

In the reduction of a dienone of Formula Ia-l to a dienol of Formula Ia-2 any keto group in the side chain symbolized by Y is simultaneously reduced and any acyloxy group is hydrolyzed, in both cases yielding a corresponding hydroxy group. Any such side chain hydroxy group can be converted to an oxo moiety by treatment with conventional oxidizing means such as manganese dioxide. It is, however, preferable to use starting materials with etherified hydroxy moieties or ketalized oxo moieties in the side chain since these are unaffected by the reduction.

The free alcohol is recovered from the reaction mixture after treatment of the mixture with acid. The alcohol can be esterified in known manner, for example, by basecatalyzed reaction with a carboxylic acid halide or carboxylic acid anhydride. Illustrative bases include inorganic bases such as sodium hydroxide and potassium hydroxide and organic bases such as a sodium alkoxide or an amine, especially a tertiary amine, and more particularly, pyridine and the picolines.

The ketodienes of Formula Ia-l can also be converted to their 7 B-hydroxy-h-hydrocarbyl derivatives represented by the formula:

wherein Y, R R R R and m are as previously defined and R is lower hydrocarbyl by reaction of the ketodiene with a Grignard reagent of the formula:

R MgX (VII) wherein R is as previously defined and X is a halogen having an atomic number of from 17 to 35, inclusive (i.e., chlorine or bromine). This Grignard reaction is conducted in known manner. For example, the Grignard reagent is prepared by reacting a hydrocarbyl halide with magnesium in an ether reaction medium, for example, ethyl ether or tetrahydrofuran, at elevated temperature, generally in the range of from about 40 C. to about 75 C. The ketodiene (Ia-1) is then added to the Grignard solution at about room temperature, although higher or lower temperatures can be employed. The resulting reaction product is hydrolyzed to produce the free alcohol, which can be esterified as discussed above.

Alternatively, the alcohols can be prepared by reaction of ketodiene (Ia-1) with a hydrocarbyl alkali metal compound such as methyl lithium, sodium acetylide, potassium acetylide, and the like.

If a dienone of Formula Ia-1 is to be converted to a diene of Formula Ia-3 then a starting material of Formula Ia-l wherein the side chain Y includes an oxo group should not be used. Also, during the course of such conversion any ester moieties present in the side chain Will be hydrolyzed.

Illustrative examples of the dienes represented by Formulae Ia-2 and Ia3 include 3,6a13-diethyl-7,8-hydroxy-2,3,5,6,6a,8-hexahydro-1H- cyclopenta [f] [l]benzopyran,

3-ethyl-6aB-methyl-7fl-acetoxy-2,3,5,6,6a,8-hexahydro- 1H-cyclopenta[f l]benzopyran, 3-ethyl-6a 3-methyl-7{i-benzoyloxy-Z,3,5,6,6a,8-hexahydro- 1H-cyclopenta[f] 1 Jbenzopyran, 3-ethyl6a,8,7a-dimethyI-ZB-hydroxy-2,3,5,6,6a,8-hexahydrolH-cyclopenta [f 1]benzopyran, 3,7a-diethyl-6aB-methyl-7 3-hydroxy-7 u-ethyl-2,3,5,6,6a,8-

hexahydro 1H-cyclopenta[f] 1]benzopyran, 3-ethyl-6aB-methyl-7B-hyroxy-7u-viny1-2,3 ,5 ,6,6a,8-hexahydrolH-cyclopenta [f] l benzopyran, 3-ethyl-6afl-methyl-7/3-hydroxy-7 a-ethynyl2,3,5,6,6a,8-

hexahydrolH-cyclopenta [f] 1]benzopyran,

3 -ethyl-6a,8,7a-dimethyl-7fi-acetoxy-2,3,5 ,6 ,6a,8-hexahydro-1H-cyclopenta[f] [l]benzopyran,

3- (4-oxopentyl -6aB-methyl-7fl-hydroxy-23,5,6,6a,8-

hexahydrolH-cyclopenta [f 1]benzopyran,

3 4-oxopentyl) -6aB-ethyl-7fi-hydroxy-2,3,5,6,6a,8-hexahydro-1H-cyclopenta[f l 1 benzopyran,

3-[ (4,4-ethylenedioxy) pentyl] -6a,B-methyl-7fi-hydroxy- 2,3,5 ,6,6a,8-hexahydro- 1H-cyclopenta[f [l]benzopy 3- [4,4-( 2',3 -butylenedioxy-pentyl] -6a;8-ethyl-7fl-hydroxy-2,3,5,6,6a,8-hexahydro-lH-cyclopenta[f] [1] benzopyran,

3- 4-t-butoxypentyl -6a 8-methyl-7fl-hydroxy-2,3,5,6,6a,8-

hexahydro-lH-cyclopenta[f] 1]benzopyran,

3- 4-t-butoxypentyl -6a/3-ethyl-7fl-hydroxy-2,3,5,6,6a,8-

hexahydro-1H-cyclopenta[f] l benzopyran,

3- (4-hydroxypentyl) -6afi-methyl-7fl-hydroxy-2,3,5,6,6a,8-

hexahydro-lH-cyclopenta[f] 1] benzopyran and the like.

The second step of the general synthesis of the tricyclic compounds of this invention comprises conversion of the dienes of Formula Ia to the monoenes of Formula Ib by catalytic hydrogenation. Suitable catalysts include the noble metals, such as platinum, palladium, rhodium, and the like, as Well as Raney nickel and other hydrogenation catalysts. These catalysts can be employed in the form of the metal alone, or can be deposited on suitable support materials, such as carbon, alumina, calcium carbonate, barium sulfate, and the like. Palladium and rhodium are preferred as catalysts. The hydrogenation is preferably conducted in the presence of inert solvents such as hydrocarbons, alcohols, ethers, and the like. The reaction conditions of pressure and temperature are not narrowly critical, and normally a hydrogen pressure of about one atmosphere and a temperature of about room temperature are employed. These ambient conditions are generally preferred to avoid significant hydrogenation of the 4a,9b(l0b)-double bond, although more severe conditions, for example, up to about 100 C. and up to about 100 atmospheres, can be employed if desired. The hydrogenation medium can be acidic, neutral, or basic, as may be desired, although neutral media, such as hydrocarbons, e.g., toluene or hexane, or basic media, such as an alcohol-base, e.g., methanol-sodium hydroxide, mixture are preferred for best results. In general, hydrogenation of the diene of Formula Ia leads to the corresponding monoene of Formula Ib. However, in the event R is an unsaturated hydrocarbyl radical, the hydrogenation, in addition to hydrogenating the ring double bond, also hydrogenates the hr-hydrocarbyl substituent, converting it to an alkyl group.

Via the aforesaid catalytic hydrogenation C/D-trans compounds are formed in a major proportion when hydrogenating a diene of Formula Ia2. This method thus provides an advantageous synthesis of C/D-trans steroidal materials. When hydrogenating a diene of Formula Ia-l, C/D-cis compounds are formed in a major proportion. This method thus provides an advantageous synthesis of C/D-cis steroidal materials.

Compounds wherein Z is carbonyl, as represented by the formula:

R12 lb-l wherein Y, R R R and m are as previously defined,

can be converted to the corresponding alcohols or esters of the formula:

YCHg

2 R12 Ib2 wherein Y, R R R R and m are as previously defined,

or to the 7fl-hydroxy-7u-hydrocarbyl compounds of the formula:

wherein Y, R R R R R and m are as previously defined,

the synthesis of steroids having the C/D-trans-orientation, this technique is not particularly desirable. Although the ratio of ,6- to u-orientation falls to about 111 at neutral conditions when hydrogenating a compound wherein Z is carbonyl, it is preferred to hydrogenate a 7/3-alcohol or ester of Formula Ia-2 because the products of this hydrogenation are predominently the 6a/9a(l0a)-transcompounds. Compounds of Formula Ia-3 when subjected to the hydrogenation yield a ratio of )8- to worientation in between that of the compounds of Formula Ia-l and that of the compounds of Formula Ia2. When monoenes of Formula Ib1 having C/Dtrans configuration are desired, it is preferable to first reduce the dienone of Formula Ia-l to a corresponding hydroxy compound of Formula Ia2 prior to the catalytic hydrogenation. Following the catalytic hydrogenation the carbonyl moiety in Formula Ib-l can be regenerated by conventional means, such as oxidation with chromium trioxide.

The monoene compounds of Formula Ib prepared by the above-described hydrogenation contain at least three asymmetric centers, at positions 3, 6a and 9a When m is one and at positions 3, 6a and 1021 when m is two. With respect to these three centers there are thus eight antipodal configurations possible. By virtue of the unique asymmetric induction of this invention, proceeding from a racemic starting material of Formula II, Ila or IIb only four of these antipodes of Formula Ib are prepared and proceeding from an optically active starting material of Formula II, Ila or llb only two of these antipodes of Formula Ib are prepared. Moreover, by the above-described hydrogenation of this invention and by appropriate selection of the 7substituent in the diene of Formula Ia subjected to the hydrogenation there can predominently be prepared the desired 6a,9a(10a)-trans-stereo-configuration. Thus, the eventual obtention of themore desired 1313 C/ D trans configuration in the ultimate steroidal products is rendered more facile by the stereoselective reaction provided by this invention.

Illustrative examples of the monoenes of Formula Ib include 3,6aB-dimethyl-2,3,5,6,6a,8,9,9a-octahydrocyclopenta[f] l 1 -benzopyran-7 l H) -one, 3,6afl-diethyl-2,3,5,6,6a,8,9,9a-octahydrocyclopenta[f] [1]-'benzopyran-7 l H)-one 3,6aB-dipropyl-2,3,5,6,6a,8,9,9a-octahydrocyclopenta [f] [l]-benzopyran-7(1H)-one, 3,Gafi-dimethyl--hydroxy-2,3,5,6,6a,8,9,9a-octahydro- 1H-cyclopenta[f] [l]-benzopyran, 3,6afi-dimethyl-7 S-acetoxy-Z,3,5,6,6a,8,9,9a-octahydrolH-cyclopenta f] {l]benzopyran, 3,6a,8,7a-trimethyl-7/i-hydroxy-2,3,5,6,6a,8,9,9a-octahydro-1H-cyclopenta[f][1]be'nzopyran, 3,6afl,7a-triethyl-7e-hydroxy-2,3,5,6,6a,8,9,9a-octahydrolH-cyclopenta [f] l]benzopyran, 3,6aB-dimethyl-l,2,3,5,6,6a,8,9,10,IOa-decahydro- 7H-naphtho- [2, 1 -b pyran-7-one, 3-(4oxopentyl)-6a,5-methyl-2,3,5,6,6a,8,9,9aoctahydrocyclopenta[f] [l]benzopyran-7 lH)-one, 3-(4-oxopentyl)-6afl-ethyl-2,3,5,6,6a,8,9,9a-octahydrocyclopenta[f] [l]-benzopyran7( 1H) -one, 3-[(4,4-ethylenedioxy)pentyl]-2,3,5,6,6a,8,9,9a-octahydrocyclopenta [f l]benzopyran-7 1H -one, 3-(4-t-butoxypentyl)-2,3,5,6,6a,8,9,9a-octahydrocyclopenta[f] [l]benzopyran-7(1H)-one, 3- (4-hydroxypentyl -2,3 ,5, 6,6a,7, 8,9,9a-octahydrocyclopenta[f] [1]-benzopyran-7(lH)-one, and the like.

The final reaction of applicants general process for the compounds of this invention is the conversion of the monoene of Formula 1b to the perhydro compound of Formula Ic by reaction of the monoene with a compound having the formula:

(VIII) That is, the monoene of Formula Ib is reacted with water, a primary alcohol, or a carboxylic acid. This reaction is catalyzed by mineral or organic acids, for example, hydrochloric acid, phosphoric acid, sulfuric acid, paratoluenesulfonic acid, and the like. Sulfuric acid is the preferred acid catalyst, and water the preferred reactant. Although not necessary, it is desirable to conduct this reaction in the presence of an added solvent, particularly in the event the compound of Formula VIII is water. In this case, it is desirable to employ a solvent which is both miscible with Water and a solvent for the monoene of Formula Ib. Solvents of this nature include acetone, tert.- butanol, dioxane, and the like. The reaction temperature is not critical, and ambient temperature is normally employed, although higher and lower temperatures could be employed if desired.

In addition to the addition of the R OH compound, this step effects the conversion of a ketalized side chain such as the 3-[(4,4-alkylenedioxy)pentyl]-group, if present, to the 3-(4-oxopentyl)-group.

As with the compounds of Formulae Ia1 and Ib1, the compounds of general Formula Ic wherein Z is carbonyl:

YCH:

Ic-l

wherein Y, R R R R and m are as previously defined,

are readily converted to their corresponding alcohols:

wherein Y, R R R R R and m are as. previously defined,

or the fi-hydroxy-ot-hydrocarbyl compounds:

wherein Y, R R R R R R and m are as previously defined,

by the previously described methods.

In a modification of the general technique outlined above, one can simultaneously effect the hydrogenation and hydration steps, for example, by hydrogenation of a diene of Formula Ia in aqueous sulfuric acid. When this simultaneous hydrogenation-hydration reaction is effected, it is preferred to begin with a diene having a hydroxyl group in the 7B-position.

Illustrative examples of the compounds falling within the scope of Formula Ic include 3 ,6afi-dimethyl-4a-hydroxyperhydrocyclopenta [f] [1] benzopyran-7-one,

3,6a,8-dimethyl-4a-acetoxyperhydrocyclopenta [f] [1] benzopyran-7-one,

3,6afi-dimethyl-4a-methoxyperhydrocyclopenta [f] [1] benzopyran-7-one,

3,6afi-diethyl-4a-hydroXyperhydro-cyclopenta [f] l] benzopyran-7-one,

3- [4,4- 2,3 butylenedioxy-pentyl] -6aB-ethyl-4a-hydroxyperhydrocyclopenta [f l benzopyran-7-one,

3, 6aB-dimethyl-4a,7[3-dihydroxyperhydrocyclopenta [f] 11benzopyran,

3,621,8,7ottrimethyl-4a,7fi-dihydroxyperhydrocyclopenta [f] [l]benzopyran,

3 ,6aB-dimethyl 4a,7B-di acetoxy perhydrocyclopenta [f [l]benzopyran,

3 ,6aB-dimethyl-4a,7,B-di (acetoxy) perhydro-3H- naphtho [2, l-b] pyran,

3, 4-oxopentyl -6afi-methyl-4a-hydroxyperhydrocyclopenta [f] [11benzopyran-7-one,

3- 4,4-ethylenedioxy pentyl] -6aB-methyl-4a-hydroxyperhydrocyclopenta [f] [l]benzopyran-7-one,

3- (4-t-butoxypentyl -6aB-methyl-4a-hydroxyperhydrocyclopenta [f] [l]benzopyran-7-one,

3- 4-hydroxypentyl -6afi-methyl-'4a-hydroxyperhydrocyclopenta [f] 1]benzopyran-7-one, and the like.

Although in the various compounds of Formula I, as well as their precursors of Formulas II, 11a and 11b, the symbol Y comprehends a 3-oxobutyl moiety, it should be noted that it is not preferred to work directly with such oxo-substituted compounds. This is because in many of the reaction steps utilized herein such an oxo moiety would itself be affected. Accordingly, it is preferred to protect such an oxo moiety and regenerate the oxo moiety from its protected form at any desirable stage of the reaction sequence. Protection of the 0x0 moiety can be effected according to means known per se. Similarly, regeneration of the 0x0 moiety from its protected form can be effected by means known per se. Thus, one preferred method of effecting protection of the 0x0 moiety is to convert it to its ketal by reaction with an alkanediol in a known manner. Advantageous results are obtained Where protection of the 0x0 moiety is effected at an early stage in the synthesis. An especially preferred alkanediol is butanediol which affords excellent resistance to attack by nucleophilic reagents. When butanediol is employed, R and R when taken together are for example, 2,3- butylenedioxy. Similarly, an oxo moiety can be converted to its dithia ketal by reaction with dithioethane in a known manner, for example, in acetic acid at room temperature and in the presence of boron trifluoride. Moreover, a monothia ketal can similarly be prepared in a known manner, for example, by reaction of the oxo moiety with Z-mercaptoethanol in dioxane at room temperature in the presence of zinc chloride and sodium sulfate. Also, the monoaza ketals can be prepared in a known manner, for example, by reaction of the 0x0 moiety with Z-hydroxyethylamine in the presence of acid. Finally, the 0x0 moiety can be reduced to the corresponding hydroxy compound which can then be etherified or esterified. As indicated above, the 0x0 moiety can be regenerated from its protected form at any desired stage of the reaction sequence. Thus, it can be readily produced by hydrolysis of the alkylenedioxy ketals in a known manner. Similarly, it can be regenerated from the dithio ketal in a known manner, for example, by treatment with phenylmercuric chloride and calcium carbonate in ethanol or by treatment with dioxane in methanolic hydrochloride. Also, it can be regenerated from a monothia ketal in a known manner, for

example, by treatment under strong acidic conditions, for example, by treatment with aqueous sulfuric acid in dioxane or hydrochloride in acetic acid. Moreover, it can be regenerated from a monoazo ketal in a known manner, for example, by treatment with a strong aqueous acid. Also, ethers and/or esters can be reconverted to the free hydroxy group which in turn can be oxidized to give the x0 moiety.

As indicated above, the tricyclic compounds of this invention are useful as intermediates for the preparation of various steroid compounds, depending upon the nature of Y. For example, compounds wherein Y is R and R is hydrogen or alkyl lead to 95,10a-steroids or wot-steroids, whereas compounds wherein R is 3-substituted-butyl, lead to l9-nor-steroids of the normal series, as illustrated by the following reaction scheme.

In the first step of this reaction scheme, the compound of Formula Ic is oxidized to form bicyclic compound of the Formula X wherein Y is hydrogen, alkyl, 3-alkylenedioxybutyl, the monothia, monoaza or dithia chalcogen thereof, or etherified 3-hydroxybutyl, by contact with such oxidizing agents as chromic acid, potassium dichromate, or potassium permanganate. Jones reagent (chromic acid, sulfuric acid an acetone), or a chromic acid-acetic acid mixture are preferred as oxidizing agents. The nature of Z is unchanged in this reaction, except when Z is hydroxymethylene [-CH(OH)]. In this instance, unless the hydroxyl group is protected, as by formation of a lower acyl ester, it is oxidized to form a carbonyl group.

Similar oxidation is effected when compound (Ic) contains as Y a B-hydroxybutyl group. A hydroxylated product is readily obtained, however, by hydrolysis of a product ester. The reaction temperature is not narrowly critical, and temperatures in the range of from 0 C. to about C. are suitable, although ambient temperatures are preferred.

In the second step, bicyclic compound (X) is treated with acid or base to effect cyclization to (XI). In this reaction, it is preferred that the water of reaction be removed, as by refluxing the reaction mixture with an emotroping agent in the presence of a strong acid and separating the water from the condensate. Mitable strong acids are sulfuric acid, p-toluenesulfonic acid, potassium bisulfate and the like. Alternatively, base catalyzed dehydration can be utilized, for example, by refluxing compound (X) in the presence of methanolic sodium hydroxide.

The hydrogenation of cycle-olefin XI to tricyclic compounds XV or XII is preferably effected with a noble metal catalyst, e.g., a palladium-charcoal catalyst or a rhodium catalyst. In Formula XV R represents hydrogen or alkyl. Thus, when compounds of Formula XI wherein Y represents hydrogen or alkyl are hydrogenated, compounds of Formula XV are obtained, whereas when compounds of Formula XI wherein Y represents a 3-substituted butyl radical of the formula hydrogenation yields compounds of Formula XII. Hydrogenation products of Formula XI are converted to retorsteroids by base catalyzed reaction with ethyl vinyl ketone to yield a 9e,l0ot-androst-4-ene-3-one of Formula XVIII. The conversion of compounds of Formula XI to compounds of Formula )0] and of the latter compounds of Formula XVIII are described in greater detail in Belgian Pat. No. 663,197.

Compounds of Formula XI wherein Y is R can also be directly reacted with methyl vinyl ketone yielding a 5- hydroxy-tetracyclic compound of Formula XVI. These latter compounds can then be subjected to dehydration followed by hydrogenation or to hydrogenation followed by dehydration to yield 95,10mor wot-steroids of Formulas XVII and XVIII. These procedures are described in greater detail in Netherlands Octrooiaanvrage No. 6412939. Still other methods of utilizing compounds of Formula XI are described in the literature and other patents.

In those compounds of Formula XI wherein Y is a 3- substituted butyl radical, catalytic hydrogenation over a noble metal catalyst such as palladium gives a 19-nor-4,5- seco compound of Formula XII. The 3-substituted butyl radical is then converted to a 3-oxobutyl radical, thus giving a compound of Formula XIII.

The conversion of the S-substituted butyl radical of the compound of Formula XII to the 3-oxobutyl radical of the compound of Formula XIII can be efiected for each particular meaning of R and R in a mann'er known per so as described hereinabove for generation of a 3-0xobutyl moiety in compounds of Formula I. When R and R taken together are alkylenedioxy, the conversion, of compounds of the Formula XII to compounds of the Formula XIII proceeds directly in the presence of acid, e.g. hydrochloric acid or sulfuric acid and acetone at room temperature.

However, the conversion of the 3-substituted butyl radical of the compounds of Formula XII to the 3-oxo-butyl radical of the compounds of Formula XIII for other specified values of R and R defined hereinafter, proceeds through a reaction sequence which yields novel intermediates. When R taken alone is CR R is lower alkyl (i.e., when R is lower alkoxy) and R taken alone is hydrogen; compounds of Formula XII, for example, l0[3- tertiary-bu-toxy-butyl] l8 methyl l9 nor desA an- 17 drostan-5,17-dione, can be converted by cyclization to a novel class of enol ethers of the formula:

H2)m R1 H I (XIX) wherein R R R R and R are each independently hydrogen or lower alkyl; R is a primary alkyl group of from 1 to 5 carbon atoms and m is an integer having a value of from 1 to 2.

Exemplary of the compounds of this formula is 6a,9atrans-2-methyl-6aB-ethyl-7-oxo 3,4,5a,10a,5,6,8,9,10,11- decahydro 2H,7H cyclopenta[5,6]naptho[2,1-b]pyran. Cyclization to the enol ether of Formula XIX can be effected by treatment of the alkoxy substituted derivatives of Formula XII with acid and heat in a solvent such as for example, benzene or toluene. Suitable acids for the cyclization step are p-toluenesulfonic acid, hydrochloric acid, phosphoric acid, potassium bisulfate and the like. This cyclization is preferably effected at the reflux temperature of the reaction medium although temperatures from between 70 to 130 are suitable.

Further reaction of this novel class of enol ethers by hydration with acid such as sulfuric acid, hydrochloric acid, p-toluenesulfonic acid and the like, in a solvent at room temperature yields hydroxy-ether compounds of the formula:

wherein R R R R and R are each independently hydrogen or lower alkyl; R is a primary alkyl group of from 1 to 5 carbon atoms and m is an integer having a value of from 1 to 2.

Suitable solvents for this reaction include acetone, methanol, ethanol and the like. Exemplary of compounds of this formula is 6a,9a-trans-Z-methyl-6at3-ethyl-7-oxo-perhydrocyclopenta[5,6]naphtho[2,1-b]pyran-11a-ol.

The hydroxy-ether compounds can be further reacted by an oxidation process to produce the diketone compounds of structure XIII. Moreover, where R in Formula XX is ethyl, i.e., 6a,9a-trans-2-methyl-6afl-ethyl-7-oxo-perhydrocyclopenta[5,6]naptho[2,1-b]pyran-lla-ol, the oxidation' reaction affords the novel compound 10 [3-oxo-butyl1- 1S-methyl-19-nor-desA-androstan-5,17-di0ne. Exemplary of the suitable oxidizing agents for the reaction are chromic acid and potassium dichromate. Jones reagent (chromic acid, sulfuric acid and acetone) is an especially preferred reagent for this purpose. The reaction is carried out in the presence of a mineral acid such as hydrochloric acid or sulfuric acid at room temperature.

Cyclization of the compounds of Formula XIII can then be effected to yield 19-nor-androst-4-ene-3-one of the Formula XIV. The cyclization reaction of compounds of Formula XIII to compounds of Formula XIV can be effected by treatment of the compound of Formula XIII with acid or base. In this reaction it is preferred that the water of reaction be removed, as by refluxing the reaction mixture with an azeotroping agent in the presence of a strong acid and separating acid, p-toluenesulfonic acid, potassium bisulfate and the like. Alternatively, basecatalyzed dehydration can be utilized, for example, by heating compound XIII in the presence of methanolic sodium hydroxide or potassium t-butylate in t-butanol to about 50 C. Moreover, where R in Formula XIII is ethyl, for example, l0-[3-oxo-butyl]-18-methyl-19-n0r desA-androstan-S,17-dione, the cyclization process yields the novel l8-homo di-keto compound 13fi-ethyl-gon 4-ene- 3,17-dione.

Compounds of Formula XIV can be selectively alkynylated by a suitable organo metalic acetylide affording norgestrel 13,8-ethyl-l7u-ethinyl-17-hydroxy-gon-4-ene-3- one). Exemplary of the suitable alkynylating agents to effect the conversion to norgestrel are the alkali acetylides such as lithium acetylide, potassium acetylide, sodium acetylide, etc. The reaction is carried out in the presence of liquid ammonia in a suitable solvent system such as for example, benzene or toluene. The alkynylation is effected preferably at the reflux temperature of the reaction medium although temperatures from between -60 to 30 are suitable. Exemplary of other suit ble reagents to effect the acetylenic addition are lithium acetylide ethylenediamine complex in a dimethylform amide solvent and Grignard analogs such as mono and his acetylene-magnesium halides. The acetylene addition, known with l3-methyl-substituted steroids, is similarly effected with the more bulky 13-ethyl-substituted steroid notwithstanding the increased steric hindrance in the latter configuration.

The above and other methods for utilizing compounds of Formulas XII and XIII as intermediates in syntheses of steroidal materials are described in published patents and in the literature, such as French Pat. Nos. 1,364,556; 1,452,898; 1,432,569 and 1,465,400.

Compounds of Formulas XI, XIV, XVII, XVIII, XIX

and XX wherein Z is carbonyl can be converted into corresponding pregnane compounds, i.e., compounds in which Z is of the formula by known procedures. Thus, for example, 19-nor-14B androst-4-ene-3,17-dione can be converted into 19-nor- 14,9,l7a-progesterone and desA-androst-9-ene-5,17-dione can be converted into desA-pregn-9-en-5-one. These procedures for converting androst-l7-ones into pregnanes are best effected if all carbonyl groups other than that in the 17-position are initially protected.

As has been pointed out above, the products of this invention are produced in the form of various optically active antipodes, which can be carried through the entire reaction sequence, or which can be resolved at suitable places during the reaction sequence. For example, at any stage wherein a compound having a secondary hydroxyl group is present, such as hydroxytetrahydropyran (IV), or any of the hydroxy compounds of Formula I, one can react the secondary alcohol with a dicarboxylic acid to form a half-ester. Suitable dicarboxylic acids in clude lower alkyl dicarboxylic acids such as oxalic acid, malonic acid, succinic acid, glutamic acid, adipic acid, or aromatic carboxylic acids such as phthalic acid. The resulting half-ester is then reated with an optically active base, such as 'brucine, ephedrine, or quinine, to produce a diastereomeric salt. The salts, after separation, are then readily reconverted to optically active alcohols. As an alternative, the secondary alcohol can be reacted with. an optically active acid, for example, camphorsulfonic acid. The resulting diastereomeric esters are then separated and reconverted to the alcohols.

It is preferred hat the resolution be effected at some stage in the synthesis of alken-3-one, as by the abovementioned resolution of hydroxytetrahydropyran (IV). In a more preferred technique, optically active -alkyl- 5-valerolactone is obtained from S-alkyl-S-oxopentanoic acid via known microbiological processes. The S-form of this lactone is the preferred form for use in accordance with this invention. In a third method, the racemic lactone can be hydrolyzed to the corresponding hydroxy. acid, which is then resolved by treatment with an optically active base in the manner described above. Still other methods will be apparent to those skilled in the art. Resolution at such early stages in the overall process described herein is highly preferred because of the improved efficiency in the production of steroids having a desired stereo-configuration. Because the stem-configuration is retained throughout the synthesis of alken-3-one (l1), and further, because the condensation of alken-3 one or variant (II, Ila or 'IIb) with cycloalkanedione (D1) is stereo-specific, one, by proper selection of stereo-isomers at these early stages, can ensure that substantially all of the tricyclic compounds of this invention and the steroids derived therefrom have a selected stereoconfiguration. Thus, by this technique, the production of compounds of the undesired configuration is minimized or prevented entirely, with an attendant increase in the efliciency of the production of compounds of the desired configuration.

In the claims, all compounds shall be construed to include, independently, the racemic form of the compound and independently, each enantiomeric form, i.e., the d and l configurations unless specifically indicated otherwise.

The following examples are illustrative. All temperatures are in degree centigrade and all products having centers of asymmetry are racemic unless specifically indicated otherwise.

EXAMPLE 1 (a) A mixture of 38 grams of Z-methylcyclohexane- 1,3-dione, 51 grams of sodium hydroxide, and 450 milliliters of water was hydrogenated over Raney nickel catalyst at a maximum temperature of 140 C. and a maximum pressure of 750 p.s.i. The reaction mixture was filtered and the filtrate, containing sodium S-hydroxyheptanoate, was acidified with concentrated hydrochloric acid (to a pH of l), and then refluxed for 30 minutes. The resulting solution was cooled and filtered. The filtrate was extracted with three l-liter portions of benzene and the combined benzene extracts, after Washing with water, drying over sodium sulfate, and evaporation, yielded 26 grams of 5-ethyl-5-hydroxyvaleric acid lactone.

(b) A suspension of 5.2 grams of lithium aluminum hydride in 250 milliliters of anhydrous ether was added with stirring over one hour to a solution of 64 grams of S-ethyI-S-hydroxyvaleric acid lactone in 500 milliliters of anhydrous ether maintained at 0-5 C. and under a nitrogen atmosphere. After the addition of 250 milliliters of 3 N sulfuric acid, the reaction mixture was extracted with three lO-O-milliliter portions of ether. The combined ether extracts were then washed with two IOO-milliliter portions of sodium bicarbonate solution, then with two ISO-milliliter portions of water. After drying over sodium sulfate, the etheric solution was evaporated at 45 C. under vacuum to yield 57.1 grams of 6-ethyl-2-hydroxytetrahydropyran.

(c) To 7.6 grams of magnesium in 7 milliliters of anhydrous tetrahydrofuran containing a few drops of ethyl bromide and a few milligrams of iodine maintained at 4548 C. was added, over a four-hour period, 120 milliliters of a 20.8 weight percent solution of vinyl chloride in tetrahydrofuran. The resulting reaction mixture was cooled to 30 C. and a solution of 13 grams of 6-ethyl- Z-hydroxytetrahydropyran in 40 milliliters of tetrahydrofuran was added. After standing overnight, there was added ice and ammonium chloride. Extraction of the resulting mixture with three 250-milliliter portions of ether, washing with three IOU-milliliter portions of sodium chloride, drying over sodium sulfate, and evaporation gave 16 grams of 3,7-dihydroxy-l-nonene.

(d) To a solution of 25 grams of l-nonene-3,7-diol in 1250 milliliters of 1,2-dichloroethane was added 0.25 gram of hydroquinone and 300 grams of manganese dioxide. The resulting slurry was stirred vigorously for one hour without heating, during which time the reaction temperature rose to about 30 C. The resulting reaction mixture was filtered and the manganese dioxide filter cake was washed thoroughly with 500 milliliters of 1,2- dichloroethane. The combined filtrates were evaporated in vacuo at 40 C. to yield 17.3 grams of 7-hydroxy-1- nonen-3-one. This compound is reacted with hydrogen chloride to produce 1-chloro-7-hydroxynonan-3-one, with dirnethylamine to produce l-'(N, N-dimethylamino)-7-hydroxy-3-one, with water to produce 1,7-dihydr0xynonan- 3-one, or with ethanol to produce l-ethoxy-7-hydroxynonan-3-one.

EXAMPLE 2 Employing procedures similar to those described in Example 1, except that cyclohexane-l,3-dione is substituted for 2 methylcyclohexane-1,3-dione, 7-hydroxyoctl-en-3-one is produced.

EXAMPLE 3 Employing procedures similar to those described in Example 1, except that 2-ethylcyclohexane-1,3-dione is substituted for Z-methylcyclohexane-1,3-dione, 7-hydroxydec-l-en-3-one is produced.

EXAMPLE 4 A 20 percent solution of diisobutyl aluminum hydride in 31.4 milliliters of toluene was added over a 30-minute period to a solution of 5 grams of L -5-pentyl-5- hydroxy-valeric acid lactone in 50 milliliters of toluene at 70 C. After wonkup of the resulting reaction mixture as described in Example l(b), there was obtained 5 grams of practically pure optically active 6-pentyl-2- hydroxytetrahydropyran.

To a solution of this product in 20 milliliters of tetrahydrofuran was added at 30 C. a solution of vinyl magnesium chloride in tetrahydrofuran prepared from 3.5 grams of magnesium and excess vinyl chloride in the manner described in Example 1(c). After hydrolysis of the reaction product with an ammonium chloride-ice mixture, followed by extraction with ether, there was obtained 5.72 grams of 3(R,S-),7(sl -dihydroxy-l-dodecene as an oil. After crystallization from isopropyl etherpentane at 0 C., the diol melted at 65.5-67.5 C. and had an optical rotation [a] =+5.9 as determined from a 1 percent solution in chloroform.

A solution of 5.22 grams of the diol in 1,2-dichloroethane was stirred with 63 grams of manganese dioxide in the presence of 50 milligrams of hydroquinone for one hour. After filtration to remove the manganese dioxide, washing with additional dichloroethane and ether, and evaporation of the filtrate at 30 C., there was obtained 3.98 grams of optically active 7(S)-hydroxy-1-dodecen- 3-one.

EXAMPLE 5 A solution of racemic 7-hydroxy-l-nonen-3-one [21.3 g.; crude obtained as in Example 1(d)] in hexane (200 ml). was treated for 15 hours at 25 C. with a solution of ()-ot-phenylethylamine (11.5 g.) in hexane ml). The reaction mixture was then purified by chromatography on alumina (660 g.). Elution with hexane first gave unpolar by-products. Hexane-ether-(4:1)-, (1:1), and straight ether then eluted 2-[2-(l-phenylethylamino) ethyl]-6-ethyl-2tetrahydropyranol obtained in solid form after evaporation of the solvents.

21 EXAMPLE 6 To a solution of the 2[2-(l-phenylethylamino)ethyl]- 6-ethyl-Z-tetrahydropyranol (prepared and purified as described in Example 5; 13.87 g., purified by chromatography) in dioxane (200 ml.) a solution of oxalic-acid (4.55 g.; 99.0% anhydrous powder) in dioxane (215 ml.) was added. After standing at 25 C. (65 hours) the precipitated White crystals were filtered off and washed with cold dioxane ml.). Thus, the oxalate of 2-[2-(1- phenylethylamino ethyl] 6 (S -ethyl-Z-tetrahyrlropyranol was obtained, M.P. 123428", [a] =28.2 (c.=l.0; methanol).

EXAMPLE 7 To 20 g. of magnesium turnings in a 500 ml. flask equipped with Dry Ice condenser, thermometer, and dropping funnel, ml. of tetrahydrofuran was added followed by dropwise addition of vinyl chloride solution (200 ml.; 26% solution in tetrahydrofuran) while the oil bath in which the flask was immersed was maintained at 70. The vinyl chloride was added at such a rate so that the reaction temperature remained at 4652. Iodine vapor and methyl iodide were used to initiate the reaction.

Upon completion of the addition of the vinyl chloride, the reaction mixture was cooled to 5, and 6-[4,4- (ethylenedioxy)-pentyl]-tetrahydrofuran-2-ol (44.63 g.) dissolved in 150 ml. tetrahydrofuran was added dropwise to the Grignard reagent at 5 to 0. The resulting mixture was stirred overnight at room temperature.

The solution was then treated with ice and ammonium chloride solution (200 ml.), and the mixture extracted three times, each time with 500 ml. of chloroform. The organic phase was washed once with ammonium chloride solution and twice with water, and then dried over anhydrous sodium sulfate. Removal of the solvent in vacuo afforded crude 11,11-ethylenedioxy-3,7-dihydroxy-1-dodecene as pale yellow liquid which solidified upon refrigeration.

A sample of the crude product was recrystallized once from isopropyl ether-hexane to give clusters of colorless needles which upon three addition recrystallizations from the same solvent provided 11,11-ethylenedioxy-3,7-dihydroxy-l-dodecene which melted at 52-54.

A solution of 22.0 g. of 11,1l-ethylenedioxy-l-dodecene-3,7-diol in benzene (600 ml.) and diethylamine ml.) was treated under vigorous stirring with manganese dioxide (108 g.) at 25 C. After stirring for 18 hours at room temperature, the manganese dioxide was filtered oil and washed with benzene. After evaporation of the filtrate crude Z-(Z-diethylaminoethyl)-6-(4,4-ethylenedioxypentyl)-2-tetrahydropyranol was obtained.

A sample of the crude 11,11-ethylenedioxy-3,7-dihydroxy-l-dodecene (10 g.; prepared as described above) was dissolved in dichloroethane (250 ml.), and to this solution activated manganese dioxide g.) was added. The mixture was stirred for 1 hour at room temperature, filtered, and the filter cake washed three times, each time with 250 ml. of dichloroethane. Concentration of the combined filtrate afforded crude 11,11-ethylenedioxy- 7-hydroxy-1-dodecen-3-one.

EXAMPLE 8 A mixture of 8.3 grams of 7-hydroxy-1-none-3-one, 7 grams of 2-methylcyclopentane-1,3-dione, 0.1 gram of hydroquinone, 4.2 milliliters of pyridine, and 42 milliliters of toluene was refluxed 'under a nitrogen atmosphere for two hours employing a Dean-Stark water-collection apparatus. The reaction solution, after cooling, was filtered to recover unreacted 2-methylcyclopentane-l,3- dione. The filtrate was evaporated to dryness, yielding 9.78 grams of crude 3-ethyl-6a,8-methyl-2,3,5,6,6a,8-hexahydrocyclopenta[f] [1]benzopyran-7(1H)-one. A mixture of the crude product, 1 gram of charcoal, and 100 milliliters of ether was refluxed for 5 minutes. After decantation, the resulting solution was concentrated and 20 milliliters of hexane was added to cause crystallization. A first crop of 3.88 grams was obtained which melted at 96-99" C. After concentration of the mother liquor and crystallization from a cold diisopropyl ether/hexane mixture, a second crop of crystals melting at 100-103 C. was obtained.

Employing similar procedures 3-ethyl-6aB-methyl-2,3, 5,6,6a,8 hexahydrocyclopenta[f] [1]benzopyran-7(1H)- one is prepared by substituting l-chloro-7-hydroxynonan- 3-one for the 7-hydroxy-1-nonen-3-one.

EXAMPLE 9 A mixture of 16.2 grams of 7-hydroxy-l-nonen-3-one, 11.5 grams of 2-methylcyclopentane-1,3-dione, 210 milliliters of xylene, and milliliters, of acetic acid was refluxed for 1% hours. After evaporation, the crude reaction product, Weighing 27.9 grams, was extracted with two -millillter portions of benzene. The remaining residue, which weighed 1.7 grams, was unreacted 2-methylcyclopentane-1,3-dione. The benzene extracts were combined and evaporated to yield 25 grams of crude product. A solution of this product in hexane was filtered through alumina and, after evaporation of the hexane and crystallization of the product from a hexane-pentane mixture, there was obtained 16.6 grams of 3-ethyl-6aB-methyl-2,3, 5,5,6a,8 hexahydrocyclopenta[f][1]benzopyran-7(1H)- one, melting point 104106 C.

Employing similar procedures 3-ethyl-6aB-methyl-2,3, 5,6,6a,8 hexahydrocyclopenta[f] [1]benzopyran-7(1H)- one is prepared by substituting 1-(N,N-dimethy1amino)- 7-hydroxynonan-3-one for the 7-hydroxy-l-nonen-3-one.

EXAMPLE 10 A mixture of 1.56 grams of 7-hydroxy-l-nonen-3-one, 1.12 grams of 2-methylcyclopentane-l,3-dione, and 50 milliliters of toluene was refluxed for 6 hours. Workup of the reaction mixture in the manner described in Example 9 yielded 3-ethyl-'6afl-methyl-2,3,5,6,6a,8-hexahydrocyclopenta [f] l]benzopyran7 1H) -0ne.

EXAMPLE 1 1 A mixture of 1.56 grams of 7-hydroxy-l-nonen-3-one, 1.12 grams of 2-methylcyclopenta-1,3-dione, 16 milliliters of p-dioxane, and 80 milligrams of p-toluenesulfonic acid was reacted at 25 C. for 22 hours. Employing the Work-up procedures of Example 9, there was obtained 3-ethyl-6afi methyl 2,3,5,6,6a,8 hexahydrocyclopenta [f] [l]benzopyran-7(lH)-one.

EXAMPLE 12 A mixture of 1.56 grams of 7-hydroxy-1-nonen-3-one, 1.12 grams of Z-methylcyclopentane-l,3-dione, 0.16 gram of p-toluenesulfonic acid, and 16 milliliters of benzene was refluxed for 30 minutes and worked up as described in Example 9 to yield 3 ethyl-6a/3-methyl-2,3,5,6,6a,8- hex ahydrocyclopenta [f] [1]benzopyran-7 1H -one.

EXAMPLE 13 A mixture of 1.56 grams of 7-hydroxy-1-nonen-3-one, 1.12 grams of Z-methylcyclopentane-l,3-dione, 16 milliliters of toluene, 0.8 milliliter of pyridine, and 0.16 gram of p-toluenesulfonic acid was refluxed for 30 minutes. After treatment of the reaction mixture as described in Example 9, there was obtained 3-ethyl-6afi-methyl-2,3,5,6, 6a,8-hexahydrocyclopenta [f] [1]benzopyran-7(1H)-one.

EXAMPLE 14 A mixture of 1.56 grams of 7-hydroxy-1-nonen-3-one, 1.12 grams of 2-methylcyclopentane-1,3-dione, 25 milliliters of toluene, 5 milliliters of cyclohexanone, and 0.3 gram of aluminum isopropoxide was refluxed under a nitrogen atmosphere for one hour. After workup as described in Example 15 below, there was obtained 3-ethyl- 621B methyl 2,3,5,6,6a,8 hexahydrocyclopenta[f] [1] benzopyran-7(1H)-one, melting point 100-102 C.

23 EXAMPLE 15 A mixture consisting of 1.56 grams of 7-hydroxy-1- nonen-3-one, 1.12 grams of 2 methylcyclopentane-1,3- dione, and 0.16 gram of potassium acetate in tert.-butanol, was held at 25 C. for 20 hours. The resulting reaction mixture was extracted with three ZOO-milliliter portions of ether. After washing each ether extract with three l -milliliter portions of Water, the ether extracts were combined, dried over sodium sulfate, filtered, and evaporated to dryness. The residue which weighed 2.16 grams was dissolved in 22 milliliters of hexane and chromatographed on alumina. After elution with hexane and evaporation of the hexane, there was obtained spiro[4amethyl 7a-hydroxy 2,3,4,4a,5,6,7,7a octahydrocyclopenta[b]pyran 5-one-2,2'-(6' ethyltetrahydropyran)], melting point 88-95" C. The melting point was 9397 C. after sublimation at 65 C. and 0.01 mm.

EXAMPLE 16 A mixture of 0.3 gram of 7-hydroxy-1-nonen-3one, 0.3 gram of Z-methylcyclopentane-1,3-dione, 6 milliliters of tert.-butanol, and 15 milligrams of sodium hydroxide was held at 25 C. for 2% days yielding spiro[4a-methyl- 7a-hydroxy 2,3,4,4a,5,6,7,7a octahydrocyclopenta[b] pyran-5-one-2,2 (6-ethyltetrahydropyran)]. The same product was obtained when potassium hydroxide was substituted for sodium hydroxide.

EXAMPLE 17 EXAMPLE 18 A mixture of 100 milligrams of spiro[4a-methyl-7a-hydr0xy-2,3,4,4a,5,6,7,7a octahydrocyclopenta[b]pyran-S- one-2,2-(6'-ethyltetrahydropyran)], 5 milliliters of henzene, and milligrams of p-toluenesulfonic acid was held at 25 C. for 20 hours. After workup in the manner described in Example and crystallization from hexane, there was obtained pure 3-ethyl-6aB-methyl-2,3,5,6, 6a,8-hexahydrocyclopenta[f] 1]benzopyran-7( 1H)-one.

EXAMPLE 19 Employing apparatus and procedures similar to those described in Example 8, except that 2-ethylcyclopentane- 1,3-dione is substituted for 2 methylcyclopentane-1,3- dione, there is produced 3,6afi-diethyl-2,3,5,6,6a,8-hexahydrocyclopenta[f][l]benzopyran-7(1H)-one melting at 57-59 C.

EXAMPLE 20 Employing apparatus and procedures similar to those described in Example 8, but substituting Z-methylcyclohexane-1,3-dione for 2 methylcyclopentane 1,3-dione, there is produced S-ethyl 6a/3-methyl 1,2,3,5,6,6a,8,9- octahydro 7H naphtho[2,1-b1pyran-7-one melting at 91-92 C.

EXAMPLE 21 Employing apparatus and procedures similar to those described in Example 8, but substituting Z-ethylcyclo-hexane-l,3-dione for Z-methylcyclopentane-l,3-dione, there is produced 3,6afi-diethyl 1,2,3,5,6,6a,8,9 octahydro-7H- naphtho [2,1-b]pyran-7-one.

EXAMPLE 22 Employing apparatus and procedures similar to those described in Example 8, but substituting 7-hydroxy-1- octen-3-one for 7-hydroxy-1-nonen-3-one, there is produced 3,6216 dimethyl 2,3,5,6,6a,8-hexahydrocyclopenta [f] [1]benzopyran-7(1H)-one.

24 EXAMPLE 23 Employing apparatus and procedures similar to those described in Example 19, but substituting 7-hydroXy-1- octen-3-one for 7-hydroxy-1-nonen-3-one and 2-ethylcycl0pentane-1,3-dione for Z-methylcyclopentane-1,3-dione, there is produced 6afi-ethyl-3-methyl-2,3,5,6,6a,8-hexahydrocyclopenta[f1 ['l benzopyran-7 1 H -one.

EXAMPLE 24 Employing apparatus and procedures similar to those described in Example '8, but substituting 7-hydroxy-ldecen-3-one for 7-hydroxy-l-nonen-3-one, there is produced 6a5-methyl-3-propyl-12,3,5,6,6a,8-hexahydrocyclopenta [f] ljbenzopyran 7 1H)-one.

EXAM PLE 25 A mixture of the optically active 7-hydroxy-1-dodecen- 3-one, obtained as described in Example 4, 2.25 grams of 2-methylcyclopentane-1,3-dione, 50 milliliters of xylene, and 25 milliliters of acetic acid was refluxed for 1 /2 hours under a nitrogen atmosphere. After evaporation under vacuum, the residue was extracted with cold benzene leaving 400 milliliters of unreacted methylcyclopentanedione as an insoluble residue. The benzene solution was then evaporated to yield 5.56 grams of optically active 3 pentyl 6aB-methyl-2,3,5,6,6a,8-hexahydrocyclopenta[f][1]benzopyran 7(11-1) one. After chromatographing the crude product on alumina, followed by crystallization from methanol-water, the product melted at 6061 C., [a] 166.

EXAMPLE 26 A mixture of Z-methyl-cyclopentane-1,3-dione (2.24 g.), xylene ml.) and glacial acetic acid (25 ml.) was refluxed under a nitrogen atmosphere for 2 minutes. Then optically active 2- [2-( l-phenylethylamino ethyl] -6-ethyl- 2-tetrahydropyranol oxalate (6.4 g., prepared as described in Example 6) was added and the mixture refluxed for 1 hour. The resultant solution was then washed with H O (2X 50 ml.), saturated NaHCO solution (2X 50 ml.) and H 0 (1X 50 ml.). The aqueous phases were extracted with benzene (2x 1111.). The combined benzene and xylene fractions were evaporated and the residue (2.4 g.) was chromatographed on alumina g.). With hexane and hexan-ether-(1911) (total 13 fractions, 160 ml. each) pure 3-ethyl-6a,8-rnethyl-2,3,5,6,6a,S-hexahydro cyclopenta[f][1]benzopyran 7(1H) one was eluted. After evaporation of fractions 3-11 (pure by thin layer chromatography analysis) yellow crystals resulted. This product had a rotation of [111 145.3 (o -1.0; CHCI Recrystallization from pentane afforded 3-ethyl- 6a,8 methyl 2,3,5,6,6a,8 hexahydro-cyclopenta[f][1] benzopyran-7(1H)-one as beige crystals, M.P. 97100, -145.7 (c.=1.0; CHCl EXAMPLE 27 A mixture of Z-methyl-cyclopentane-1,3-dione (8.6 g.), xylene (157 ml.) and glacial acetic acid (78.5 ml.) was refluxed under a nitrogen atmosphere for 2 minutes. Then a solution of crude 2-(Z-diethylarninoethyl)-6-(4,4-ethylenedioxypentyl)-2-tetrahydropyranol (23 g., as prepared in Example 7) in xylene (78.5 ml.) was added to the reaction mixture during 15 minutes. The mixture was then refluxed for another 15 minutes and then worked up by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo to give a crude mixture of 3- (4,4-ethylenedioxypentyl) -6a,8-methyl 2,3,5,-6,6a,8-hexahydrocyclopentafi] [1]benzopyran 7(1H) one, 4 (3- hydroxy 7 oxooctyl) 7aB methyl-2,3,5,6,7,7a-hexahydroindene 1,5(1H) dione and 3 (4 oxopentyl)-6afimethyl 2,3,5,6,6a,8 hexahydrocyclopenta[f][1]benzopyran-7(1H)-one. This mixture was separated by chromatography on alumina. Elution with hexane afforded 3- (4,4 ethylenedioxypentyl) 6afl methyl 2,3,5,6,6a,8-

25 hexahydrocyclopenta[f][1]benzopyran-7(1H) one, elution with hexane-ether (9:1) afforded 3-(4-oxopentyl)- 6afi methyl 2,3,5,6,6a,8 hexahydrocyclopenta[f][l] benzopyran-7(lH)-one and elution with chloroform afforded 4- 3-hydroxy-7-oxooctyl) -7a/3-methyl-2,3 ,5 ,6,7,7ahexahydroindene-l,5(1H)-dione, an oil.

A mixture of pure 3 (4,4 ethylenedioxypentyl)-6a;8- methyl 2,3,5,6,6a,8 hexahydrocyclopentafi][1]benzopyran-7( 1H)-one (2.86 g.), acetone (56 ml.) and 1 N sulfuric acid (5.6 ml.) was allowed to stand at room temperature for 18 hours. The workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and Water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gave a crude mixture of 4-(3-hydroxy- 7 oxooctyl 7afi methyl 2,3,5,6,7,7a hexahydroindene 1,5(1H) dione and 3 (4 oxopentyl) 6a,8- methyl 2,3,5,6,6a,8 hexahydrocyclopenta[f] [l]benZopyran-7(1H)-one. This mixture was separated by chromatography on silica gel (52 g.). Elution with hexaneether (1:1) and ether alone afforded pure 3-(4-oxopentyl) 6a,8 methyl 2,3,5,6,6a,8 hexahydrocyclopenta [f] [1]benzopyran-7(1H)-one (M.P. 74-85 C., thin layer chromatography, one spot). Elution with methanol afforded 4-(3-hydroxy-7-oxooctyl)-7a;8-methyl-2,3,5,6,7,7ahexahydroindene 1,5 (1H) dione as an oil (thin layer chromatography, one spot).

A mixture of 4-(3-hydroxy 7 oxooctyl)-7a,6-methyl- 2,3,5,6,7,7a hexahydroindene 1,5(1H) dione (1.7 g.), benzene (50 ml.) and p-toluenesulfonic acid (170 mg.) was refluxed for 2 hours. The workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gave crude 3 (4 oxopentyl)-6afl-methyl 2,3,5,6,6a,8 hexahydrocyclopenta[f][1]benzopyran- 7(lH)-one which was chromatographed on alumina (46.5 g.). Elution with hexane-benzene (9:1), (4:1), (1:1) and (1:2) afforded pure 3-(4-oxopentyl)-6afl-methyl 2,3,5,6,6a,8 hexahydrocyclopenta[f] [1]benzopyran- 7(lH)-one (thin layer chromatography, one spot).

EXAMPLE 28 A solution of 3-(4-oxopentyl)-6a/8-methyl-2,3,5,6,6a,8- hexahydrocyclopenta [f] [1]benzopyran-7(1H)-one (1.3 6 g.) in tetrahydrofuran (60 ml.) was added at C. within minutes to a mixture of lithium aluminum hydride (262 mg.) in tetrahydrofuran (40 ml.), with stirring and under a nitrogen atmosphere. The reaction mixture was stirred for an additional 30 minutes at 0 C. and then worked up by careful addition of water, filtration and evaporation of the filtrate in vacuo at 38 C. yielding crude 3-(4-hydroxypentyl) 6a,? methyl-2,3,5,6,6a,8- hexahydrocyclopenta[f][1]benzopyran 7 (1H)-o1 (thin layer chromatography, one spot) was obtained.

EXAMPLE 29 A solution of 1 gram of 3ethyl-6afi-rnethyl-2,3,5,6,6a,8- hexahydrocyclopenta[f][1]benzopyran-7(1H)-one in milliliters of tetrahydrofuran was added over a 15-minute period to a stirred mixture of 100 milligrams of lithium aluminum hydride in milliliters of tetrahydrofuran maintained at 0 C. and under a nitrogen atmosphere. After stirring at 0 C. for an additional hour, a few drops of concentrated sodium hydroxide solution was added. The resulting solution was filtered and evaporated to give 0.982 ram of 3-ethyl6afl-methyl-2,3,5,6,6a.8-hexahydrocyclopenta[f] [11benzopyran-7 (1H)-o1 melting at 107- 109 C.

Treatment of 235 milligrams of the product for 20 hours at room temperature with 0.28 gram of benzoyl chloride in 2 milliliters of pyridine as a solvent yielded the 7-benzoate ester.

Treatment of 200 milligrams of the product alcohol for ethyl-6a/3-methyl 2,3,5,6,6a,8 hexahydrocyclopenta[f]- [1]benzopyran-7(1H)-one, 3,6afl diethyl 2,3,5,6,6a,8- hexahydrocyclopenta [f] [1]benzopyran-7(1H)-ol is produced.

EXAMPLE 31 In a manner similar to that described in Example 29, 3,6afl-dimethyl 2,3,5,6,6a,8 hexahydrocyclopenta[f]- [1]benzopyran-7(1H)-one is reduced to its corresponding alcohol.

EXAMPLE 32 Employing procedures similar to those described in Example 29, 6aB-methyl-3-propyl-2,3,5,6,-6a,8-hexahydrocyclopenta[f] [1]benzopyran 7(-1H) one is reduced to 6a5-methyl-3-propyl 2,3,5,6,6a,8 hexahydrocyclopenta- [f] [1]benzopyran-7(1H)-ol.

EXAMPLE 33 Employing procedures similar to those described in Example 29, except that 3-ethyl-6a,8-methyl-1,2,3,5,6,6a, 8,9 octahydro-7H-naphtho[2,1-b]pyran-7-one is substituted for the 3-ethyl-6aB-rnethyl-2,3,5,6,6a,8-hexahydrocyclopenta[f] [11benzopyran 7(1H) one, there is produced 3-ethyl-6afl-methyl l,2,3,5,6,6a,8,9 octahydro- 7H-naphtho[2,1-b]pyran-7-ol.

EXAMPLE 34 In a manner similar to that described in Example 29, 3,6ap-diethyl-1,2,3,5,6,6a,8,9-octahydro 7H naphtho- [2,1-b]pyran-7-one is reduced to its corresponding alcohol.

EXAMPLE 35 A solution of a 137-gram portion of the optically active enol ether produced as described in Example 25 in 25 milliliters of tetrahydrofuran was added over a 15- minute period to a stirred solution of 137 milligrams of lithium aluminum hydride in 30 milliliters of tetrahydrofuran at 0 C. and under a nitrogen atmosphere. After stirring for an additional 30 minutes at 0 C. and the addition of water to the reaction mixture, the mixture was worked up in the manner described in Example 29 to yield 1.39 grams of optically active 3-pentyl-6aB*methyl-2,3,5, 6,6a,8-hexahydrocyclopenta [f] 1]benzopyran7 1H)-o1.

EXAMPLE 3 6 A solution of crude 3-(4-hydroxypentyl)-6a[3-rnethyl- 2,3,5,6,6a,8 hexahydrocyclopenta[f][1]benzopyran-7- (1H)-o1 (1.52 g., obtained by the procedure of Example 28) in pyridine (15 ml.) was treated with acetic acid anhydride (7.5 ml.) at room temperature. After standing at room temperature for 20 hours the reaction mixture Was treated with methanol (23 ml.) at 0 C. After standing for 30 minutes it was then extracted with benzene (3 times), the combined benzene extracts were washed with H O, in HCl, saturated NaHCO solution and again with H O. The benzene extract was then evaporated and the residue purified by chromatography on alumina (53.4 g.). Elution with hexane and hexane-ether (9: 1) afforded pure 7fi-acetoxy-3(4-actoxypentyl)-6a/3-methyl 2,3,5,6,6a,8- hexahydro-lH-cyclopenta[f][1]benzopyran (thin layer chromatography, one spot).

EXAMPLE 37 A mixture of 11,11-ethylenedioxy-1-dodecene-3,7-diol (5.0 g.), acetone ml.) and 1 N sulfuric acid (25 ml.) was stirred at room temperature for 6 hours. After the workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo crude 11-oxo-1-dodecene-3,7-diol was obtained. This crude product was used for the next step.

A solution of crude 11-oxo-1-dodecene-3,7-diol (3.3 g.) in 1,2-dichlorethane (100 ml.) was treated with vigorous stirring with manganese dioxide (20 g.) at room temperature. After stirring for 1 hour, the manganese dioxide was filtered oif and washed with 1,2-dichlorethane. After evaporation of the combined l,2-dichlorethane filtrates, crude 7-hydroxy-1-dodecene-3,1l-dione was obtained. This crude product was used for the next step.

A mixture of crude 7-hydroxy-l-dodecene-3,ll-dione (2.6 g.), 2-methyl-cyclopentane-1,3-dione (1.6 g.), toluene (35 ml.), pyridine (3.5 ml.) and hydroquinone (60 mg.) was refluxed with stirring for 2 /2 hours. After extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo crude 3-(4-oxopentyl) 6a,/3 methyl-2,3,5,6,6a,8-hexahydrocyclopenta[f] [l]benzopyran-7(lH)-one was obtained. This material was chromatographed on alumina (90 g.). Fractions (90 ml. each) were taken as follows. 14=hexane-benzene (4:1), 8=hexane-benzene (2:1) and 9-12=hexane-benzene (1: l). Fractions 3-12 afforded 3- (4-oxopentyl)-6a5-methyl 2,3,5,6,6a,8 hexahydrocyclopenta[f][11benzopyran- 7(1H)-one.

EXAMPLE 38 10.0 grams of 3,7dihydroxy-1-nonene was dissolved in 300 milliliters of benzene and milliliters of diethylamine (dist) To this solution there was added, under vigorous stirring, at C., 60.0 grams of activated manganese dioxide. The reaction mixture was then stirred at 25 C. for 15 hours and then at 45 C. for 5 hours. The manganese dioxide was then filtered off and the filtrate washed three times, each time, with 50 milliliters of benzene. It was then evaporated to dryness at 45 C. in vacuo yielding a crude product which by thin layer chromatography (Thin layer chromatography system: Silica gel G plates, solvent benzene:diethylamine 9:1, detection 50 percent aqueous para-toluenesulfonic acid solution, 120 C. Visibility of desired product l-diethylamino-7-hydroxy-nonan-3one can be improved with I vapors) was shown to contain mainly 1diethylamino- 7-hydroxy-nonan-3-one plus traces of unreacted starting material 3,7-dihydroxy-l-nonene. This crude product was used without purification for the next step.

6.47 grams of Z-methylcyclopentane-1,3-dione, 130 milliliters of xylene and 65 milliliters of glacial acetic acid was refluxed for 5 minutes. To the resulting solution there was added under refluxing and a nitrogen atomsphere during 15 minutes a solution of 13.25 grams of the above-prepared crude product containing l-diethylamino-7-hydroxy-nonan-3-one dissolved in 65 milliliters of xylene. The reaction mixture was then refluxed for 15 minutes. The resultant solution was then cooled to 25 C. with an ice bath and then twice washed, each time with 100 milliliters of H 0, then twice, each time with 100 milliliters of saturated Nal-ICL, solution and then again with 100 milliliters of H 0. The aqueous phases were twice extracted, each time with 200 milliliters of benzene. After evaporation of the combined xylene and benzene solutions at 55 C. in vacuo, light brownish crystals were obtained which were diluted with 50 milliliters of hexane, filtered off after 5 minutes, and twice washed, each time with 15 milliliters of hexane, yielding dl-3-ethyl-2,3,5,6,6a,8-hexahydro Gap methylcyclopenta[f][1}benzopyran-7(lH)-one (melting point 101- 102.5 C., beige crystals were obtained). Further quantities of this product were obtained from the mother liquor by evaporation to dryness and column chromatography using aluminum oxide (40 grams, activity grade III) and hexane as a solvent.

28 EXAMPLE 39 To a freshly prepared solution of methyl lithium (0.2 g.) in tetrahydrofuran (50' ml.), a solution of 3-ethyl 2,3,5,6,6a,8 hexahydro6afi-methylcyclopenta[f] [1]benzopyran-7(1H)-one (1.0 g.) in tetrahydrofuran (20 ml.) is added at 20 C. within 20 minutes with stirring and under a nitrogen atmosphere. After stirring for 1 hour at 50 C., the reaction mixture is poured onto ice (50 g.) ammonium chloride (5 g.). Extraction with ether (3 portions, 200 ml. each), washing with water (3 times, 50 ml. each), drying over sodium sulfate, filtration and evaporation gives 3-ethyl 7a,6a;3 dimethyl2,3,5,'6,6a,8- hexahydrocyclopentaIf] [l]benzopyran-7(1H)-ol which is further purified by preparative thin layer chromatography on silica gel plates.

EXAMPLE 40 A solution of =6a,9a-trans-3-ethyl-2,3,5,6,6a,8,9,9a-octahydro-6a/3-methylcyclopenta[f] 1]benzopyran-7( 1H) 01 (1.5 g.) in dimethylformamide (15 ml.) is slowly (30 minutes) added at 25 to a stirred mixture of chromic acid (0.3 g.), dimethylforrnamide (30 ml.) and concentrated sulfuric acid (1 drop). Stirring is continued for another 3 hours. After work-up (extraction of the reaction mixture with benzene, washing with water and evaporation of the benzene extracts in vacuo at 40) 6a,9a trans 3 ethyl 2,3,5,6,6a,8,9,9a-octahydro-6afimethylcyclopenta[f][llbenzopyran 7(1H) one is obtained as an oil. After purification by column chromatography on aluminum oxide, this ketone is dissolved in tetrahydrofuran (25 ml.) and added (within 25 minutes) to a solution of lithium acetylide (prepared from 0.11 g. of lithium wire and acetylene in 50 ml. of liquid ammonia) at 35 C. with stirring. Stirring is then continued for 15 hours at the reflux temperature of the reaction mixture. After adding dry ammonium chloride (3 g.) and ether (50 ml.) to the reaction mixture, the ammonia was allowed to evaporate. After washing the ether phase with water (3 times), followed by filtration and evaporation in vacuo at 40, 6a,9a-trans-7a-ethinyl- 3-ethyl-2,3,5,6,6a,8,9,9a-octahydro Gap methylcyclo penta[f][l]benzopyran-7(lH)-ol is obtained as an oil. Purification is achieved by thick layer chromatography on silica gel plates.

EXAMPLE 41 A mixture of 4.9 grams of 3-ethyl-6af3-methyl-2,3,5,6, 6a,8 hexahydrocyclopenta [f] [1]benzopyran 7(1H) 01 produced in a manner similar to that described in Example 29, milliliters of methanol, 1.6 milliliters of 3 N sodium hydroxide, and 0.8 gram of 5 percent palladium-on-charcoal was stirred at room temperature in a nitrogen atmosphere. The hydrogen uptake stopped after 2 hours at which time 520 milliliters had been adsorbed. After addition of 0.3 milliliter of acetic acid, the catalyst was filtered off and the filtrate evaporated to dryness to yield 4.9 grams of product consisting predominantly of 6a,9a-trans 3 ethyl-6a/3-methyl-2,3,5,6,6a,8,9,9a-octahy drocyclopenta [f] [1]benzopyran-7( 1H ol.

EXAMPLE 42 Employing apparatus and procedures similar to those described in Example 41, but substituting the product of Example 30 for that of Example 29, there is produced predominantly 6a,9a-trans-3,6afl-diethyl2,3,5,6,6a,8,9,9aoctahydrocyclopenta [f] l ]benzopyran-7( 1H)-o1.

EXAMPLE 43 Employing apparatus and procedures similar to those described in Example 41 but substituting the product of Example 31 for that of Example 29, there is produced predominantly 6a,10a trans-3,6aB-diethyl-1,2,3,5,6a,8,9, 9a-octahydrocyclopenta [f 1 l benzopyran-7 1H)-o1.

29 EXAMPLE 44 EXAMPLE 45 Employing apparatus and procedures similar to those described in Example 41 but substituting the product of Example 33 for that of Example 29, there is produced predominantly 6a,10a-trans-3-ethyl-6ap-methyl-1,2,3,5,6,

6a,8,9,10,10a-decahydro-7H-naphtho [2,1-b]pyran-7-ol.

EXAMPLE 46 Employing apparatus and procedures similar to those described in Example 41 but substituting the product of Example 34 for that of Example 29, there is produced predominantly 6a,10a trans-3,6afl-diethyl-1,2,3,5,6a,8,9, 10,10a-decahydro-7H-naphtho[2,1-b]pyran-7-ol.

EXAMPLE 47 The dienol of Example 35 was dissolved in 50 milliliters of toluene and then hydrogenated as described in Example 41, employing 378 milligrams of a percent palladium-on-charcoal catalyst. After the uptake of 111 milliliters of hydrogen, there was obtained 1.39 grams of optically active 6a,9a-trans-3-pentyl6a,8-methyl-2,3,5,6,6a, 8,9,9a-octahydrocyclopenta [f] [1]benzopyran-7(1H)-ol.

EXAMPLE 48 A solution of 7,8-acetoxy-3-(4-acetoxypentyl)-6a[3-meth yl-2,3,5,6,6a,8-hexahydro-lH-cyclopenta [f] [1] benzpyran (1.31 g.; chromatognaphed) in toluene (50 ml.) was hydrogenated under normal conditions using 5 percent palladium-on-charcoal catalyst (262 mg). The uptake (88 ml.) of hydrogen stopped after 5 hours. The workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gave crude 6a,9a-trans-7;8- acetoxy-3-(4-acetoxypentyl) 6a,8 methyl-2,3,5,6,6a,8,9, 9a-octahydro-lH-cyclopenta[f] [1]benzopyran as an oil.

EMMPLE 49 A solution of 11,1l-ethylenedioxy-7-hydroxy-1-dodecen- 3-one (6.7 g.; prepared as described in Example 7) in 25 ml. of toluene was treated with 2-methyl-1,3-cyclopentadione (3.50 g.), pyridine (2.7 ml.), and hydroquinone (140 mg), and the resulting mixture refluxed for 8 hours using Dean-Stark apparatus.

At the end of the reflux period, the reaction mixture was chilled, the 2-methyl-1,3-cyclopentadione removed by filtration, and the filtrate concentrated in vacuo (water aspirator, 40). The crude product 3-[4,4-(ethylenedioxy)penty1] 2,3,5,6,6a,8 hexahydro 6afl methylcyclopenta[f] [1]benzopyran-7(1H)-one (8.15 g.) was chromatographed on 82 g. of neutral alumina Grade III and the column eluted with hexane-10% ether. The eluted fractions 2-10 contained the desired product which was recrystallized once from isopropyl ether-hexane giving almost colorless crystals of 3-[4,4-(ethylenedioxy) pentyl1- 2,3,5,6,6a,8 hexahydro-SaB-methylcyclopenta[f][1]benzopyran-7(1H)-one, a sample of which upon being recrystallized from hexane-ether melted at 6972.

EXAMPLE 5 0 To lithium aluminum hydride (0.38 g.) in tetrahydrofuran (90 ml.) at 0, 21 solution of the 3-[4,4-(ethylenedioxy)pentyl]-2,3,5,6,6a,8-hexahydro 6at3 methylcyclopenta[f][l] benzopyran-7(lH)-one (3.7 g.) in 75 ml. tetrahydrofuran was added over a period of minutes.

Upon completion of the addition of 3-[4,4-(ethylenedioxy)pentyl]- ,3,5,6,6a,8-hexahydro 6afi methylcyclopenta[f][l]benzopyran 7(1H) one, the mixture was stirred at 0 for 1 hour. A small amount of water was then added to the reaction mixture. Concentration of the reaction mixture in vacuo gave a yellow liquid which crystallized upon standing. The product, 3-[4,4-(ethylenedioxy)pentyl]-2,3,5,6,6a,8-hexahydro 6a,8 methylcyclopenta[f] [l]benzopyran-7(lH)-ol, was collected by filtration.

A sample of the product, after recrystallization four times from dilute methanol and once from ether-hexane, melted at 91-93".

EXAMPLE 51 3-[4,4-(ethylenedioxy)pentyl] 2,3,5,6,6a,8 hexahydro 6a,8 methylcyc1openta[f] [1]benzopyran-7(1H)-ol (10 g.) was dissolved in 250 ml. of toluene and hydrogenated in the presence of 1.7 g. of 5% palladium-oncharcoal catalyst. During ca. 9 hour period 710 ml. of H were consumed. The hydrogenation was performed at room temperature.

At the end of the reaction period the catalyst was removed by filtration, and the solvent removed in vacuo. The crude product was chromatographed on g. of neutral alumina Grade 111, and the column eluted with hexane-ether (1:2) giving purified product, 6a,9a-trans- 3-[4,4-(ethylenedioxy)pentyl] 2,3,5,6,6a,8,9,9a octahydro 6a,8-methylcyclopenta[f] [1]benzopyran 7(1H)-ol.

EXAMPLE 52 A mixture of 465 milligrams of 3 ethyl 6a 3 methyl 2,3,S,6,6a,8 hexahydrocyclopenta[f] [l]benzopyran- 7(1H)-one, as produced in a manner similar to that described in Example 8, 20 milliliters of toluene, and milligrams of 5 percent palladium-on-charcoal was hydrogenated under ambient conditions. The hydrogen uptake ceased after one hour, after which time 50 milliliters had been adsorbed. After filtering the reaction mixture and evaporation of the filtrate, there was obtained the product monoenol ether as an oil containing predominantly 6a,9acis 3 ethyl 6a/8 methyl-2,3,5,6,6a,8,9,9a-octahydrocyclopenta[f] [1]benzopyran-7( lH)-one.

EXAMPLE 53 Employing apparatus and procedures similar to those described in Example 52 but substituting the product of Example 19 for that of Example 8, there is produced predominantly 6a,9a-cis-3,6a/3-diethyl-2,3,5,6,6a,8,9,9a octahydrocyclopenta [f] [1]benzopyran-7 1H) -one.

EXAMPLE 54 Employing apparatus and procedures similar to those described in Example 52 but substituting the product of Example 22 for that of Example 8, there is produced predominantly 6a,9a-cis-3,6aB-dimethyl 2,3,5,6,6a,8,9,9'a octahydrocyclopenta [f] [1]benzopyran-7(1H)-one.

EXAMPLE 55 Employing apparatus and procedures similar to those described in Example 47 but substituting the product of Example 23 for that of Example 7, there is produced predominantly 6a,9a-cis-6afl methyl-3-propyl-2,3,5,6,6a, 8,9,9a octahydrocyclopenta[f][1] benzopyran 7(1H)- one.

EXAMPLE 56 Employing apparatus and procedures similar to those described in Example 52 but substituting the product of Example 20 for that of Example 8, there is produced pre dominantly 6a,l0a cis 3 ethyl-6afi-methyl-1,2,3,5,6,6a, 8,9,10,10a-decahydro-7H-naphthol[2,1-b1pyran-7-one.

EXAMPLE 57 Employing apparatus and procedures similar to those described in Example 52 but substituting the product of Example 21 for that of Example 8, there is produced predominantly 6a,l0a-cis 3,6afi-diethyl 1,2,3,5,6,6a,8,9, 10, 10a-decahydro-7H-naphtho 2, 1-b pyran-7-one.

31 EXAMPLE 58 A solution of the 4.9 grams of hydrogenation product obtained in Example 41 in 100 milliliters of acetone and 15 milliliters of l N sulfuric acid was held at 25 C. for 30 minutes. After extraction of the reaction mixture with ether, washing with water and sodium bicarbonate solution, drying over sodium sulfate, filtration and evaporation, there was obtained 5.1 grams of crude 6a,9a-trans 3 ethyl 621,5 methylperhydrocyclopenta[f][11benzopyran-4a,7diol. This product on crystallization from an ether-hexane mixture at C. yielded 3.5 grams, melting point l13-116 C. A second crop weighing 0.4 gram, melting point 114l17 C., was obtained from the mother liquor. The melting point was increased from 121-1 22.5 C. after several additional recrystallizations.

By employing similar procedures but substituting methanol or acetic acid for the water, there are obtained the corresponding 4a-methoxyand 4a-acetoxy-derivatives, respectively.

EXAMPLE 59 Employing apparatus and procedures similar to those described in Example 58 but substituting the product of Example 42 for that of Example 41, there is produced predominantly 6a,9a trans 3,6a,8-diethylperhydrocyclopenta [f] [1]benzopyran-4a,7-diol.

EXAMPLE 6O Employing apparatus and procedures similar to those described in Example 58 but substituting the product of Example 43 for that of Example 41, there is produced predominantly 6a,9a trans-3,6aB-dimethylperhydrocyclopenta[f} [1]benzopyran-4a,7-diol.

EXAMPLE 61 Employing apparatus and procedures similar to those described in Example 58 but substituting the product of Example 44 for that of Example 41, there is produced predominantly 6a,9a trans -6a,8-methyl-S-propylperhydrocyclopenta [f] l ]benzopyran-4a,7-diol.

EXAMPLE 62 Employing apparatus and procedures similar to those described in Example 8 but substituting the product ot Example 45 for that of Example 41, there is produced predominantly 6a,l(la-trans-3-ethyl 621,6 methylperhydronaphtho[2,l-b]pyran-4a,7-diol.

EXAMPLE 63 Employing apparatus and procedures similar to those described in Example 58 but substituting the product of Example 46 for that of Example 41, there is produced predominantly 6a,10a-trans-3,6a;8 diethylperhydronaphtho[2,1-b]pyran-4a,7-diol.

EXAMPLE 64 A 1.25-grarn portion of the crude hydrogenation product obtained as described in Example 47 was dissolved in milliliters of acetone and treated with 1 N sulfuric acid as described in Example 58. After standing at 25 C. for 45 minutes and workup of the reaction mixture, there was obtained 1.20 grams of optically active 6a,9a-trans-3- pentyl 6ai3 methylperhydrocyclopenta[f][llbenzopyran-4a,7-diol as an oil.

EXAMPLE 65 A solution of 6-[4(t-butoxy)pentyl]-tetrahydropyran- 2-01 (5 g.) in tetrahydrofuran (50 ml.) was added within minutes at 5 to a solution of vinyl magnesium chloride in tetrahydrofuran, prepared from magnesium (2.0 g.) and 4 g. of vinyl chloride, dissolved in tetrahydrofuran (50 ml.). After stirring overnight at room temperature, the reaction solution was treated with ammonium chloride-ice and then extracted with chloroform (3 times, each time 100 ml.). The organic phase was washed with water, dried and evaporated in vacuo giving crude 3,7- dihydroxy- 1 1-(t-butoxy)-dodec-1-ene.

Crude 3,7-dihydroxy 11 (t butoxy)-dodec-1-ene (5.8 g., prepared as described above) was dissolved in benzene (200 ml.) and the reaction mixture was then stirred for 2 hours at 25 after addition of activated manganese dioxide (58 g.). Filtration and evaporation of the filtrate at 30 in vacuo afforded crude 7-hydroxy- 1l-(t-butoxy)dodec-l-en-3 -one.

EXAMPLE 66 A mixture of crude 6a,9a trans-7,8-acetoxy-3-(4-ace toxypentyl)-6afl methyl 2,3,5,6,6a,8,9,9a octahydro- 1H-cyclopenta[f][1]benzopyran (1.3 g., obtained by the procedure of Example 48), acetone (30 ml.) and 1 N sulfuric acid (15 ml.) was allowed to stand for 1 hour at room temperature. The workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo afiorded crude 6a,9a-trans-3-(4-acetoxypentyl) 7 3 acetoxy 6afl methylperhydrocyclopenta [f][1]benzopyran-4a-ol, which was purified by chromatography on alumina (67.5 g.). Fractions (68 ml. each) were taken as follows: l3 =hexane-ether (9:1), 46=hexane-ether (4:1), 7-9 =hexane-ether (2:1) and 10l2=hexane-ether (1:1). Thin layer chromatography analysis showed fractions 4-10 (1.02 g. after evaporation) to be pure 6a,9a-trans-3-(4-acetoxypentyl)-7B-acetoxy 62113 methylperhydrocyclopenta[f] [l]benzopyran- 4a-ol.

EXAMPLE 67 6a,9a trans 3 [4,4-(ethylenedioxy)pentyl]-2,3,5,6, 6a,8,9,9a-octahydro 6afi methylcyclopenta[f] [1]benzo pyran-7(1H)-ol (9.04 g., obtained and purified as de scribed in Example 51) was dissolved in acetone (180 ml.) and to this solution ml. of 1 N H 80 was added. The reaction mixture was allowed to remain at room temperature for 4 hours before it was neutralized with sodium bicarbonate solution, diluted with ca. 180 ml. of Water, and extracted three times, each time with 200 ml. of chloroform. The extract was washed three times with water, dried, and the solvent removed to aiford a slightly pink-colored, viscous liquid.

This crude product was chromatographed on 240 g. neutral aluminum Grade III, and the column eluted with hexane-ether (1:2 and 1:9) followed by chloroform-ether (1:1). Several of the eluted fractions contained the desired product, 6a,9a-trans 3 [4,4-(ethylenedioxy)pentyl] afi methylperhydrocyclopenta[f] [l]benzopyran- 4a,7,B-diol.

EXAMPLE 68 Crude 7-hydroxy 11 (t-butoxy)-dodec-1-en-3-one (4.6 g., obtained as described in Example 65) was dissolved in toluene (46 ml.) and to this solution there was added 2-methylcyclopentane 1,3 dione (3 g.), pyridine (4.6 ml.) and hydroquiuone mg). The reaction mixture was then refluxed for 8 hours, using a Dean-Stark water trap. The workup and chromatography purification was performed as described in Example 8 and afforded 3-[4-(t-butoxy)pentyl-6afi methyl 2,3,5,6,6a,8 hexahydrocyclopenta f] 1]benzopyran-7 1H)-one.

In order to remove the protective t-butylether group, a 1 g. sample of the product obtained above was refluxed for 5 hours under a nitrogen atmosphere in tetrahydrofuran (50 ml.) containing 10 ml. of l N hydrochloric acid. Workup by extraction with ether, washing with water and evaporation to dryness in vacuo at 60 yielded 3 (4 hydroxypentyl) 6afi methyl-2,3,5,6,6a,8-hexahydrocyclopenta [f] [1]benzopyran-7(1H)-one.

A sample of the so-prepared 3 (4 hydroxypentyl)- 6at3 methyl 2,3,5,6,6a,8 hexahydrocyclopenta[f][1] benzopyran-7(1H)-one (0.5 g.) was dissolved in ether (10 ml.) and reduced by reaction with a solution of lithium alumium hydride (0.1 g.) in ether (10 ml.) at

(1 hour stirring). After addition of 10 drops of water, the reaction mixture was filtered and the filtrate evaporated giving 3 (4 hydroxypentyl)-6a,8-methyl-2,3,5,6, 6a,8hexahydrocyclopenta[f] [1]benzopyran 7(1H) ol.

EXAMPLE 69 Employing apparatus and procedures similar to those described in Example 58 but substituting the ketone of Example 52 for the 7-01 of Example 41, there is produced predominantly 6a,9a cis-3-ethyl-4a-hydroxy-6a 8-methylperhydrocyclopenta[f] 1]benzopyran-7-one.

EXAMPLE 70 Employing apparatus and procedures similar to those described in Example 58 but substituting the ketone of Example 53 for the diol of Example 41, there is produced predominantly 6a,9a cis-3,afi-diethyl-4a-hydroxyperhydrocyclopenta[f] [1]benzopyran-7-one.

EXAMPLE 71 Employing apparatus and procedures similar to those described in Example 58 but substituting the ketone of Example 54 for the diol of Example 41, there is produced predominantly 6a,9a cis-3,6aB-dimethyl-4a-hydroxyperhydrocyclopenta [f [1]benzopyran-7-one.

EXAMPLE 72 Employing apparatus and procedures similar to those described in Example 58 but substituting the ketone of Example 6 for the diol of Example 41, there is produced predominantly 6a,10a cis-3-ethyl-4a-hydroxy-6aB-methylperhydronaphtho [2,1-b1pyran-7-one.

EXAMPLE 73 Employing apparatus and procedures similar to those described in Example 58 but substituting the ketone of Example 57 for the diol of Example 41, there is produced predominantly 6a,10a cis- 3,6a 3-diethyl-4a-hydroxyperhydronaphtho [2,1-b]pyran-7-one.

EXAMPLE 74 A mixture of 117 milligrams of 3-ethyl-6aB-methyl- 2,3,5,6,6a,8 hexahydrocyclopenta[f][IJbenzopyran 7 (1H)-ol produced in a manner similar to that described in Example 29, 10 milliliters of isopropanol, 5 milliliters of 1 N sulfuric acid, and milligrams of 5 percent palladium-on-charcoal was hydrogenated at standard temperature and pressure. Workup of the reaction product in the manner described in Example 58, followed by crystallization from an ether-hexane mixture, gave 64 milligrams of 6a,9a trans 3-ethyl-6afl-methylperhydrocyclopenta[f] [l]benzopyran-4a,7-diol, melting point 108 112 C.

By employing similar procedures, but substituting methanol or acetic acid for the water, there are obtained the corresponding 4a-methoxyand 4a-acetoxy-derivatives, respectively.

EXAMPLE 75 A solution of 255 milligrams of 6a,9a-trans-3-ethyl- 6ap methylperhydrocycolpenta[f] [1]benzopyran 4a,7- diol produced as described in Example 58 in 10 milliliters of dichloromethane was treated with a solution of 200 milligrams of chromic acid in 5 milliliters of 3 N sulfuric acid and stirred at room temperature for 18 hours. The resulting reaction mixture was Worked up as described in Example 5 8 to yield a crude product which was purified by chromatography on silica gel. Recrystallization of the thus-recovered product from an ether-hexane mixture yielded a sample of 6a,9a-trans-3-ethyl-4a-hydroxy-6afimethylperhydrocyclopenta [f] [11benzopyran 7-one melting at 120-121.5 C.

EXAMPLE 76 A solution of 6.88 grams of chromic acid in 3.45 milliliters of 6 N sulfuric acid was added over a 20-minute period to a stirred solution of 3.5 grams of 6a,9a-trans-3- ethyl 6a5 methylperhydrocyclopenta[f] [1]benzopyran- 4a,7-diol, produced as described in Example 58, in 143 milliliters of acetone at 25 C. After stirring the reaction mixture for an additional 2%. hours and workup as described in Example 58, there was worked up 3.0 grams of 3a,7a trans-4- 3'-oxopentyl) -7afl-methyl-perhydroidene- 1,5-dione. A solution of this product in 172 milliliters of toluene was treated with 792 milligrams of p-toluenesulfonic acid and refluxed for 3 hours using a Dean-Stark water separator. Workup and purification as described in Example 58 yielded 2.68 grams of desA-androst-9-ene- 5,17-dione, which on crystallization from an ether-isopropyl ether mixture, melted at 101102.5 C.

EXAMPLE 77 The product of Example 64 was oxidized with a mixture of 2.04 grams of chromic acid, 45 milliliters of acetone, and 10.2 milliliters of 6 N sulfuric acid in the manner described in Example 76 to yield 900 milligrams of 4- (3 ketooctyl)-7a/3-methyl-3aB-perhydroindeue-1,S-dione. This triketone was treated with 205 milligrams of paratoluenesulfonic acid in 50 milliliters of toluene as described in Example 69 to yield 790 milligrams of d -19-butyl-desA-androst-9-ene-5,17-dione. After purification by chromatographing on silica gel, the product had an optical rotation [0c] :+60.5 as determined from a 30 percent solution in chloroform.

EXAMPLE 78 To a solution of chromatographed 6a,9a-trans-3-(4- acetoxypentyl) 7,8 acetoxy 6a/3-methylperhydrocyclopenta[f] [1]benzopyran-4a-o1 (1.0 g.) in acetone (30 ml.), a mixture of chromic acid (1.0 g.) in 6 N sulfuric acid (5.3 ml.) was added at 20 C. (ice-cooling) with stirring during 5 minutes. After this addition, the mixture was stirred for another 2 hours at room temperature. The workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo afforded crude 3a,7a trans 4-(7-acetoxy-3-oxooctyl)-1B-acetoxy-7a5- methyl-perhydroindan-5-one as an oil. This product was used for the next step.

A mixture of 3a,7a-trans-4-(7-acetoxy-3-oxooctyl)-15- acetoxy-7a,8-methyl-perhydroindan-S-one (crude oxidation product; 940 mg.), benzene (20 m1.) and p-toluenesulfonic acid (94 mg.) was refluxed for 3 hours. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gave crude acetoxy 10 (3 acetoxybutyl) 19 nor desA- androst-9-en-5-one (870 mg.) which was purified by chromatography on silica gel (87 g.). Fractions (87 1113.1. each) were taken as follows: 1-3=hexane-ether (4:1), 46=hexane-ether (2:1), 7-9=hexane-ether (1:1), 10-l2=hexane-ether (1:2) and 1315=hexane-ether (1:4). Thin layer chromatographic analysis showed fractions 913 (583 mg. after evaporation) to be almost pure 17B acetoxy 10(3 acetoxybutyl) 19 nor desA- androst-9-en-5-one.

EXAMPLE 79 A solution of chromatographed 17fi-acetoxy-10-(3- acetoxybutyl) 19 nor desA androst 9 en 5 one (5 35 mg.) in absolute ethanol (30 ml.) and triethylamine (0.15 ml.) was hydrogenated under normal conditions using 5 percent palladium-on-charcoal catalyst (55 mg). The uptake (31 ml.) of hydrogen stopped after about 5 hours. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gave 17;? acetoxy 10 (3 acetoxybutyl)- 35 19 nor desA androstan one as an oil (535 mg.). This crude product was used for the next step.

A mixture of crude 17B acetoxy (3 acetoxybutyl) 19 nor desA androstan 5 one (535 mg), ethanol (20 ml.) and 20 percent sodium hydroxide solution 2.0 ml.) was allowed to stand at room temperature for 20 hours. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo afforded crude 17fi-hydroxy-10-(3-hydroxybuty1)- 19 nor desA androstan 5 one (373 mg). This crude product was used for the next step.

To a solution of crude 17,6 hydroxy 10 (3 hydroxybutyl) 19 nor desA androstan 5 one (373 mg.) in acetone ml.) a mixture of chromic acid (634 mg.) in 6 N sulfuric acid (3.2 ml.) was added over a period of 10 minutes with stirring and ice-cooling C.). After this addition the reaction mixture was stirred for another hour at room temperature. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gave crude 10-(3- oxobutyl) 19 nor desA androstan 5,17 dione (307 mg). Thin layer chromatographic analysis showed two major spots plus three minor spots. The two major spots were separated by chromatography on silica gel g.). Elution with benzene-ether (2:1) afforded a by-product. Elution with benzene-ether (1:1) atforded the desired product 10 (3 oxobutyl) 19 nor desA androstan- 5,17-dione.

EXAMPLE 80 A mixture of 10 (3 oxobutyl) 19 nor desA- androstan-5,17-dione (chromatographed product; 96.7 mg), toluene (4 ml.) and p-toluenesulfonic acid (9.7 mg.) was refluxed for 3 hours. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gave crude 19-nor-androst-4-ene- 3,17-dione. This material was purified by chromatography on silica gel (8.5 g.). Elution with benzene-ether (1:1) alforded 19 nor androst 4 ene 3,17 dione (67.2 mg.). Upon recrystallization from ether-hexane d,1-19- nor-androst-4-ene-3,17-dione melted at 156.5-158" C.

To a stirred solution of l9-nor-androst-4-ene-3,17-dione (56.8 mg; chromatographed product) in tetrahydrofuran (2.5 m1.), tri-tert.-butoxylithium aluminum hydride (105 mg.) was added at 0 C. in one portion. After stirring for 30 minutes at 0 C., the reaction mixture was worked up by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo to give rac. 19- nortestosterone as an oil. This material was chromatographed on silica gel (5.7 g.) Elution with benzene-ether (1:1) and benzene-ether (1:2) afforded rac. 19-nortestosterone which upon recrystallization from ether-hexane yielded rac. 19-nortestosterone melting at 116.5-120 C.

EXAMPLE 81 31.05 g. of 6a,9a trans 3 ethyl 6afl methylperhydrocyclopenta [f][1]benzopyran 4a,7 diol was dissolved in 490 ml. of acetone. A solution of chromium trioxide (32.4 g.) in 162 ml. of 6 N sulfuric acid was then dissolved into the resultant solution with stirring at 20 C. (cooling with ice bath) during the course of 20 minutes. After stirring the reaction mixture for 2 hours at 20 C.,- 100 ml. of water was added. The reaction mixture was then extracted with 400 ml. of benzene and then twice, each time with 300 ml. of benzene. The benzene extracts were twice washed, each time with 200 ml. of water and the combined benzene extracts were then dried over sodium Sulfate and evaporated to dryness at 45 C. in vacuo yielding 29.5 g. of 4 (3' ketopentyl)- 7a5 methyl 33a perhydroindene 1,5 dione as a yellow oil. This was dissolved in 295 ml. of benzene and treated With 2.95 g. of para-toluenesulfonic acid. This mixture was then refluxed for 3 hours, after which it was cooled to 20 C., washed twice, each time with ml. of water, twice, each time with 100 m1. of saturated sodium bicarbonate solution, and twice again, each time with 100 ml. of water. The aqueous phases were twice re-extracted, each time with ml. of benzene; the combined benzene extracts were dried over sodium sulfate. After evaporation to dryness at 45 C., crude desA- androst-'9-ene-5,l7-dione was obtained as a brown oil. This crude product was then purified by column chromatography with aluminum oxide (265 g., active Grade H1);

The first three fractions were eluted with hexane-benzene (4:1) and contained predominantly desA-androst-9-ene- 5,17-dione; fractions 4-6 were eluted with hexane-benzene (1:1) and fractions 79 with benzene. These fractions contained purified desA-androst-9-ene-5,17-dione. Fractions 4-9 were evaporated to dryness at 45 C. in vacuo and the residue was dissolved in isopropyl eth r (5 ml.) and diluted with hexane until the solution became turbid. After seeding with desA-androst-9-ene-5,17- dione, light beige crystals precipitated. The crystals were filtered off after a few hours at room temperature and washed with an equal amount of hexane-isopropyl ether (2:1) yielding d,1-desA-androst-9-ene-5,17-dione as yellow crystals melting at 1005-1020 C.

EXAMPLE 82 Employing procedures similar to those described in Example 29 but substituting 3-methyl-6aB-ethyl-2,3,5,6,6a,8- hexahydrocyclopentafi] l]benzopyran 7(1H) one for the 3 ethyl-6afi-methyl-2,3,5,6,6a,8-hexahydrocyclopenta [f] [1]benzopyran-7 (1H)-one, there is produced 3-methyl- Gap ethyl-2,3,5,6,6a,8-hexahydrocyclopenta [f] [llbenzopyran-7 1H)-o1.

EXAMPLE 83 Employing apparatus and procedures similar to those described in Example 41 but substituting the product of Example 82 for that of Example 29, there is produced predominantly 6a,9a trans-3-methyl-6a,8-ethyl-2,3,5,6,6a,8,9, Qa-octahydrocyclopenta [f [1]benzopyran-7( 1H -o1.

EXAMPLE 84 Employing apparatus and procedures similar to those described in Example 41 but substituting the product of Example 39 for that of Example 29, there is produced predominantly 6a,9a-trans-3-ethy1-6afl,7a-dimethyl-2,3,5,6,6a, 8,9,9a-octahydrocyclopeuta [f [1]benzopyran-7(1H)-ol.

EXAMPLE 85 Employing apparatus and procedures similar to those described in Example 58 but substituting the product of Example 40 for that of Example 41, there is produced predominantly 6a,9a trans-3-ethyl-7a-ethinyl-Gafi-methylperhydrocyclopenta[f] l benzopyran-4a,'7 -diol.

EXAMPLE 86 Employing apparatus and procedures similar to those described in Example 58 but substituting the product of Example 83 for that of Example 41, there is produced predominantly 6a,9a-trans-6aB-ethyl-3-methylperhydrocyclopenta [f] 1]benzopyran-4a,7-diol.

EXAMPLE 87 Employing apparatus and procedures similar to those described in Example 58 but substituting the product of Example 84 for that of Example 41, there is produced predominantly 6a,9a-trans-6a/3,7a-dimethyl-3-ethylperhydrocyclopenta [f1 [1 benzopyran-4a,7-diol.

EXAMPLE 88 Employing apparatus and procedures similar to those described in Example 76 but substituting the product qt 37 Example 59 for that of Example 58, there is prepared 3a,7a trans 4-(3oxopentyl)-7a,8-ethylperhydroindene- 1,5-dione which is then converted to 18-nor-1318-ethyldesA-androst-9-ene-5,l7-dione.

EXAMPLE 89 Employing apparatus and procedures similar to those described in Example 76 but substituting the product of Example 60 for that of Example 58, there is prepared 3a,7a trans 4-(3'-oxobutyl)-6aB-methylperhydroindene- 1,5-dione which is then converted to 19-nor-desA-androst- 9-ene-5,17-dione.

EXAMPLE 90 Employing apparatus and procedures similar to those described in Example 76 but substituting the product of Example 61 for that of Example 58, there is prepared 3a,7a trans 4-(3'-oxohexyl)Jan-methylperhydroindene- 1,5-dione which is then converted to 19-nor-10-ethyl-desA- androst-9-ene-5,17-dione.

EXAMPLE 91 Employing apparatus and procedures similar to those described in Example 76 but substituting the product of Example 62 for that of Example 58, there is prepared 4a,8a trans 5-(3-oxopentyl)-8afimethylperhydronaph thalene-l,6-dione which is then converted to desA-D-homo-9-ene-5,17-dione.

EXAMPLE 92 Employing apparatus and procedures similar to those described in Example 76 but substituting the product of Example 63 for that of Example 58, there is prepared 4a,8a trans-5-(3'-oxopentyl)Jar:-ethylperhydronaphthalene-l,6-dione which is then converted to l8-nor-13B-ethyldesA-D-homoandrost-9-ene-5,17-dione.

EXAMPLE 93 Employing apparatus and procedures similar to those described in Example 76 but substituting the product of Example 86 for that of Example 58, there is prepared 3a,7a trans 4 (3-0xobutyl)-7a,8-ethylperhydroindene- 1,5-dione which is then converted to 18-nor-19-nor-13fiethyl-desA-androst-9-ene-5,l7-dione.

EXAMPLE 94 Employing apparatus and procedures similar to those described in Example 76 but substituting the product of Example 87 for that of Example 58, there is prepared 3a,7a trans-1fi-hydroxy-4-(3-oxopentyl) -1,7a/8-dimethylperhydroindene-S-one which is then converted to 17,8-hydroxy-17a-methyl-desA-androst-9-ene-5-one.

EXAMPLE 95 3 (4oxopentyl)-2,3,5,6,6a,8-hexahydro-6aB-methylcyclopenta[f][1]benzopyran 7(1H) one (12 g.) is dissolved in 300 ml. of toluene and hydrogenated in the pres ence of 2.0 g. of 5% palladium-on-charcoal catalyst. During ca. 9 hour period 850 ml. of H is consumed. The hydrogenation is performed at room temperature.

At the end of the reaction period the catalyst is removed by filtration, and the solvent removed in vacuo. The crude product is chromatographed on 120 g. of neutral alumina Grade HI, and the column eluted with hexane-ether (1 :2) giving a purified 6a,9a-cis-3-(4-oxopentyl)-2,3,5,6,6a,8,9, 9a octahydro 6afi-methylcyclopenta[f] [1]benzopyran- 7 1H) -one.

EXAMPLE 96 A solution of 6a,9a cis 3 (3 oxopentyl) 6aB- methyl 2,3,5,6,6a,8,9,9a octahydrocyclopenta[f][1] benzopyran-7(1H)-one (680 mg.) in tetrahydrofuran (30 ml.) is added at 0 C. within 15 minutes to a mixture of lithium aluminum hydride (131 mg.) in tetrahydrofuran ml.), with stirring and under a nitrogen atmosphere. The reaction mixture is then stirred for an additional minutes at 0 C. and then worked up by careful addition of water, filtration and evaporation of the filtrate in vacuo at 38 C. yielding crude 6a,9a-cis- 3-(4 hydroxypentyl) 6a,8 methyl 2,3,5,6,6a,8,9,9a octahydrocyclopenta [f] [1]benzopyran-7 1H -01.

EXAMPLE 97 A solution of crude 6a,9a cis 3 (4-hydroxypentyl)- 6aB methyl 2,3,5,6,6a,8,9,9a octahydrocyclopenta [f][1]benzopyran-7(1H)-ol (760 mg., obtained by the procedure of Example 96) in pyridine (7.5 ml.) is treated with acetic acid anhydride (3.75 ml.) at room temperature. After standing at room temperature for 20 hours the reaction mixture is treated with methanol, (12 ml.) at 0 C. After standing for 30 minutes it is then extracted with benzene (3 times), the combined benzene extracts are washed with H O, in HCl, saturated NaHCO solution and again with H O. The benzene extract is then evaporated and the residue purified by chromatography on alumina (26.7 g.). Elution with hexane and hexane-ether (9:1) affords 6a,9a cis 7B- acetoxy 3 (4 acetoxypentyl) 6a/3 methyl-2,3,5,6, 6a,8,9,9a octahydro-lH-cyclopenta[f] [1]benzopyran.

EXAMPLE 98 A mixture of crude 6a,9a cis 7,3 acetoxy 3 (4- acetoxypentyl 6afi-methyl-2,3,5 ,6,6a,8,9,9a-octahydro-1H- cyclopenta[f][1]benzopyran (650 mg., obtained by the procedure of Example 97), acetone (15 m1.) and 1 N sulfuric acid (7.5 ml.) is allowed to stand for 1 hour at room temperature. The workup by extraction with ether (3 times), Washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo affords crude 6a,9a cis 3 (4-acetoxypentyl) 7 8 acetoxy 6a5 methylperhydrocyclopenta [f] [1]benzopyran-4a-ol, which is purified by chromatography on alumina (33.7 g.). Fractions (34 ml. each) are taken as follows: 13=hexane-ether (9:1), 46=hexaneether (4:1), 79=hexane-ether (2:1) and 1012:hexane-ether (1:1). The fractions containing 6a,9a-cis-3-(4- acetoxypentyl) 7,8 acetoxy 6a/3-methylperhydrocyclopenta[f] [1]benzopyran-4a-ol are identified by thin layer chromatography analysis.

EXAMPLE 99 To a solution of chromatographed 6a,9a cis 3 (4- acetoxypentyl) 7 8 acetoxy 6afi methylperhydrocyclopenta[f][1]benzopyran-4a-ol (500 mg.) in acetone (15 ml.), a mixture of chromic acid (500 mg.) in 6 N sulfuric acid (2.65 ml.) is added at 20 C. (ice-cooling) with stirring during 5 minutes. After this addition, the mixture is stirred for another 2 hours at room temperature. The workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo affords crude 3a,7a-cis-4-(7-acetoxy-3-oxooctyl)- 1B-acetoxy-7afi-methylperhydroindan-5-one as an oil. This product is used for the next step.

A mixture of 3a,7a cis 4 (7-acetoxy-3-oxooctyl)- lfi acetoxy 7a,8 methyl-perhydroindan-S-one (crude oxidation product; 470 mg.), benzene (10 ml.) and ptoluenesulfonic acid (47 mg.) is refluxed for 3 hours. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gives crude 17;? acetoxy 10 (3 acetoxybutyl) 19 nor 14B- desA androst 9 en 5 one which is purified by chromatography on silica gel (43.5 g.), Fractions (44 ml. each) were taken as follows: 13=hexane-ether (4:1), 4-6 hexane-ether (2:1), 79:hexane-ether (1:1), 10-12=hexane-ether (1:2), and 13-15=hexane-ether (1 :4). The fractions (242 mg.) containing 175 acetoxy- 10 (3 acetoxybutyl) 19 nor desA androst- 9-en-5-one are identified by thin layer chromatography analysis.

EXAMPLE 100 A solution of chromatographed 17,8 acetoxy-l-(3- acetoxybutyl) 19 nor 14B desA androst 9 en-S- one (217 mg.) in absolute ethanol (15 m1.) and triethylamine (0.075 ml.) is hydrogenated under normal conditions using per cent palladium-on-charcoal catalyst (27.5 mg). The uptake (16 ml.) of hydrogen stops after about 5 hours. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gives 17/3 acetoxy (3-acetoxybutyl)- 19 nor 14 8 desA androstan 5 one as an oil. This crude product is used for the next step.

A mixture of crude 175 acetoxy 1O (3-acetoxybutyl) 19 nor 14,3 desA androstan-S-one (217 mg.), ethanol (10 ml.) and 20 percent sodium hydroxide solution (1.0 ml.) is allowed to stand at room temperature for 20 hours. Workup by extraction with ether (3 times), Washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo affords crude 17B hydroxy 1O (3 hydroxybutyl) 19 nor 14,8 desA androstan-S-one. This crude product is used for the next step.

To a solution of crude 17B hydroxy 10 (3-hydroxybutyl) 19 nor 14B desA androstan 5 one (188 mg.) in acetone (7.5 ml.) a mixture of chromic acid (317 mg.) in 6 N sulfuric acid (1.6 ml.) is added over a period of 10 minutes with stirring and ice-cooling (20 C.). After this addition the reaction mixture is stirred for another hour at room temperature. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gives crude 10-(3-oxobutyl) 19 norl4fi desA androstan 5,17 dione which is purified by chromatography on silica gel g.). Elution with benzene-ether (2:1) and then with benzene-ether (1:1) gives the desired product 10-(3-oxobutyl)-19-nor-14fldesA-androstan-5,17-dione.

EXAMPLE 101 A mixture of 10-(3-oxobutyl)-l9-nor-l4fi-desA-androstan 5,17 dione (chromatographed product; 48.3 mg), toluene (2 ml.) and p-toluenesulfonic acid (4.8 mg.) is refluxed for 3 hours. Workup by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo gives crude 19 nor 14f? androst-4-ene-3,17- dione. This material is purified by chromatography on silica gel (4.4 g.). Elution with benzene-ether (1:1) affords 19'nor-14B-androst-4-ene-3,l7-dione (33.6 mg.).

To a stirred solution of 19-nor-14fi-androst-4-ene-3,l7- dione (28.4 mg.; chromatographed product) in tetrahydrofuran (1.3 ml.), tri-tert.-butoxylithium aluminum hydride (53 mg.) is added at 0 C. in one portion. After stirring for 30 minutes at 0 C., the reaction mixture is Worked up by extraction with ether (3 times), washing of the combined extracts with sodium bicarbonate solution and water, drying over sodium sulfate, filtration, and evaporation of the filtrate at 50 C. in vacuo to give rac. 19-nor-l4/3-testosterone as an oil. This material is chromatographed on silica gel (2.8 g.). Elution With benzeneether (1:1) and benzene-ether (1:2) affords rac. 19-nor- 14fl-testosterone.

EXAMPLE 102 6a,9a trans 3 [4,4 (ethylenedioxy)pentyl] 6a,6- methylperhydrocyclopenta[f] [l]benzopyran 4a,7fi diol (1.2 g.) was dissolved in acetone (12 ml.) and this solution was added within 15 minutes to a solution of chromic acid (1.0 g.) in dimethylformamide (20 ml.) containing 1 N sulfuric acid (2 ml.). The reaction mixture was then stirred for 5 hours at room temperature, poured onto ice and extracted 3 times, each time with ml. of ether. After washing with water and sodium bicarbonate solution, the ether extracts were combined, dried over sodium sulfate, filtered and evaporated to dryness in vacuo at 35 C. yielding crude 3a,7a trans-4-[7,7-(ethylenedioxy)-3- oxooctyl]-7aj3-methyl-perhydroindane-1,5-dione.

The so-obtained crude 3a,7a trans-4-[7,7-(ethylenedioxy) 3 oxooctyl]-721/3-methyl-perhydroindane-1,5- dione was cyclized by refluxing it in a solution of methanol (50 ml.) containing 5 ml. of 3 N sodium hydroxide. The reaction mixture was then stirred for 5 hours at room temperature, poured onto ice and extracted 3 times, each time with 100 ml. of ether. After washing with Water and sodium bicarbonate solution, the ether extracts were combined, dried over sodium sulfate, filtered and evaporated to dryness in vacuo at 35 yielding crude 10-[3,3- (ethylenedioxy)butyl] 19 nor-desA-androst-Q-ene-5,17- dione. This product, which exhibited a UV-absorption at 248 m (in ethanol) was purified by column chromatography on alumina (30 g.; Grade III).

EXAMPLE 103 A solution of optically active 3-ethyl-6a,8-methyl-2,3,5, 6,6a,8 hexahydrocyclopenta[f] [l]benzopyran 7(1H)- one (1.053 g.; chromatographed) in tetrahydrofuran (25 ml.; passed through a column of alumina) was added at 0 C. within 15 minutes to a mixture of lithium aluminum hydride (105.3 mg.) in tetrahydrofuran (30 ml.; passed through a column of alumina), with stirring and under a nitrogen atmosphere. The reaction mixture was then stirred for an additional 30 minutes at 0 C. and then worked up by careful addition of water, filtration and evaporation of the filtrate in vacuo at 38 yielding crude optically active 3 ethyl-fiaB-methyl 2,3,5,6,6a,8- hexahydrocyclopenta[f][l]benzopyran 7(lH)-ol (1.0 g.). Thin layer chromatography analysis (silica gel plates, solvent benzenc-ethylacetate 1:1, detection 50 percent aqueous p-toluenesulfonic acid solution, C. for 15 minutes, then iodine at room temperature) showed one major spot plus traces of starting material. The crude was then purified by chromatography on alumina (30 g.). Elution with hexane-ether (9:1) and (4:1) afforded a total of 760 mg. pure optically active 3-ethyl-6afi-methyl- 2,3,5,6,6a,8 hexahydrocyclopenta[f][ 1]benzopyran 7 (lH)-ol. The best fraction (No. 9) had a rotation of [041 --147.3 (c.=1.0; CHCl EXAMPLE 104 A solution of optically active 3-ethyl6aB-methyl-2,3, 5,6,6a,8 hexahydrocyclopenta[f] [l]benzopyran 7 (1H)- ol (700 mg; chromatographed) in toluene (30 ml.) was hydrogenated under normal conditions using a 5 percent palladium-on-charcoal catalyst (70 mg). The uptake (80 ml.) of hydrogen stopped after about 10 hours. The catalyst was filtered off and washed with toluene. After evaporation crude optically active 6a,9a trans-3-ethyl-6afimethyl 2,3,5,6,6a,8,9,9a octahydrocyclopenta[f] [l] benzopyran 7(1H)-ol (700 mg.) was obtained. Thin layer chromatography analysis showed one major spot plus several minor spots. This crude product was used for the next step.

A mixture of crude optically active 6a,9a-trans-3-ethyl 6aB methyl 2,3,5,6,6a,8,9,9a-octahydrocyclopenta[f] [l]benzopyran-7(1H)-ol (700 mg.; prepared as above), acetone (14 ml.) and 1 N sulfuric acid (7 ml.) was allowed to stand for 1 hour at room temperature. Extraction with ether or benzene (3 portions), washing with sodium bicarbonate solution and water, drying over sodium sulfate, filtration and evaporation to dryness at 45 C. in vacuo afiorded crude optically active 6,9a-trans-3-ethyl- Gafi methylperhydrocyclopenta [f] [l]benzopyran 4a,7- 01 (672 mg). Thin layer chromatography analysis (silica gel plates, solvent benzene-ethylacetate 1:1, detection 50 percent aqueous p-toluenesulfonic acid solution, 120 C. 

